• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

肾癌中lncRNAs的综合分析以发现一种新的lncRNA（）作为葡萄球菌肠毒素基因的治疗靶点。

Integrative Analysis of lncRNAs in Kidney Cancer to Discover A New lncRNA () as A Therapeutic Target for Staphylococcal Enterotoxin Gene.

作者信息

Safarpour-Dehkordi Maryam, Doosti Abbas, Jami Mohammad-Saied

机构信息

Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

出版信息

Cell J. 2020 Jul;22(Suppl 1):101-109. doi: 10.22074/cellj.2020.6996. Epub 2020 Jul 18.

DOI:10.22074/cellj.2020.6996
PMID:32779439
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7481890/
Abstract

OBJECTIVE

Bacterial toxin can cause cell death through induction of apoptosis in cancer cell lines as well as changes in the expression patterns of long non-coding RNAs (lncRNAs) and genes. In the present study, the effect of gene on ACHN cell lines was reported along with proposing a novel pathway of apoptosis in kidney cancer.

MATERIALS AND METHODS

In this experimental study, effective lncRNAs and genes were predicted from different criteria for renal cell carcinoma (RCC) by bioinformatics methods and lncRNA-miRNA-mRNA interaction was constructed; then the effect of Staphylococcus aureus tst gene on induction of apoptosis pathways on ACHN and HDF cell lines was investigated.

RESULTS

After creation of lncRNA-miRNA-mRNA interaction, changes in expression levels of lncRNA (P=0.0024) and gene (P=0.0027) were identified, as potential apoptosis biomarkers for kidney cancer, after treating ACHN cell line by pCDNA3.1 (+) compared to the empty vector. In contrast, there was no statistically significant difference in expression levels in ACHN cell (P≥0.05). In addition, transfection by pcDNA3.1 (+) could increase ACHN cell apoptosis level (P<0.0001) compared to the pcDNA3.1 (+) group; but no significant effect was observed on normal cells.

CONCLUSION

It is suggested that lncRNA LINC00847, discovered in this study, could provide a new landscape for researches aimed to determine relationship between functional lncRNA and RCC pathways. pcDNA3.1 (+) was found to increase apoptosis in the transfected cells.

摘要

目的

细菌毒素可通过诱导癌细胞系凋亡以及改变长链非编码RNA（lncRNA）和基因的表达模式导致细胞死亡。在本研究中，报告了基因对ACHN细胞系的影响，并提出了一种新的肾癌凋亡途径。

材料与方法

在本实验研究中，通过生物信息学方法从肾细胞癌（RCC）的不同标准预测有效的lncRNA和基因，并构建lncRNA-miRNA-mRNA相互作用；然后研究金黄色葡萄球菌tst基因对ACHN和HDF细胞系凋亡途径诱导的影响。

结果

构建lncRNA-miRNA-mRNA相互作用后，与空载体相比，用pCDNA3.1（+）处理ACHN细胞系后，lncRNA（P=0.0024）和基因（P=0.0027）的表达水平发生变化，被确定为肾癌潜在的凋亡生物标志物。相比之下，ACHN细胞中 的表达水平无统计学显著差异（P≥0.05）。此外，与pcDNA3.1（+）组相比，用pcDNA3.1（+）转染可提高ACHN细胞凋亡水平（P<0.0001）；但对正常细胞未观察到显著影响。

结论

本研究中发现的lncRNA LINC00847可能为旨在确定功能性lncRNA与RCC途径之间关系的研究提供新的视角。发现pcDNA3.1（+）可增加转染细胞的凋亡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/150a9a0f0cc1/Cell-J-22-Suppl1-101-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/27ce1f166f1d/Cell-J-22-Suppl1-101-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/8ad010205a4e/Cell-J-22-Suppl1-101-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/c79d7ed60a9b/Cell-J-22-Suppl1-101-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/559a83662559/Cell-J-22-Suppl1-101-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/63c4ee805ca7/Cell-J-22-Suppl1-101-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/150a9a0f0cc1/Cell-J-22-Suppl1-101-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/27ce1f166f1d/Cell-J-22-Suppl1-101-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/8ad010205a4e/Cell-J-22-Suppl1-101-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/c79d7ed60a9b/Cell-J-22-Suppl1-101-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/559a83662559/Cell-J-22-Suppl1-101-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/63c4ee805ca7/Cell-J-22-Suppl1-101-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a5/7481890/150a9a0f0cc1/Cell-J-22-Suppl1-101-g06.jpg

相似文献

1
Integrative Analysis of lncRNAs in Kidney Cancer to Discover A New lncRNA () as A Therapeutic Target for Staphylococcal Enterotoxin Gene.肾癌中lncRNAs的综合分析以发现一种新的lncRNA（）作为葡萄球菌肠毒素基因的治疗靶点。
Cell J. 2020 Jul;22(Suppl 1):101-109. doi: 10.22074/cellj.2020.6996. Epub 2020 Jul 18.
2
Impacts of the Staphylococcal Enterotoxin H on the Apoptosis and lncRNAs in PC3 and ACHN.葡萄球菌肠毒素H对PC3和ACHN细胞凋亡及长链非编码RNA的影响
Mol Gen Microbiol Virol. 2020;35(3):180-188. doi: 10.3103/S0891416820030076. Epub 2021 Jan 21.
3
Co-expression network analysis for the identification of potential prostate cancer genes and in vitro confirmation of their expression in cell model in the presence of Staphylococcal gene.共表达网络分析鉴定前列腺癌潜在基因，并在葡萄球菌基因存在的情况下通过细胞模型进行体外表达验证。
Nucleosides Nucleotides Nucleic Acids. 2024;43(3):214-229. doi: 10.1080/15257770.2023.2249544. Epub 2023 Aug 23.
4
Systematic Investigation of Immune-Related lncRNA Landscape Reveals a Potential Long Non-Coding RNA Signature for Predicting Prognosis in Renal Cell Carcinoma.免疫相关长链非编码RNA图谱的系统研究揭示了一种预测肾细胞癌预后的潜在长链非编码RNA特征。
Front Genet. 2022 Jul 4;13:890641. doi: 10.3389/fgene.2022.890641. eCollection 2022.
5
[Regulation of proliferation and apoptosis of human vascular endothelial cell by Acheron].Acheron对人血管内皮细胞增殖和凋亡的调控
Zhonghua Shao Shang Za Zhi. 2011 Apr;27(2):156-60.
6
lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis.lncRNA 00312 通过 miR-34a-5p/ASS1 轴抑制肾细胞癌的细胞增殖、侵袭并促进凋亡。
Oxid Med Cell Longev. 2020 Mar 23;2020:5737289. doi: 10.1155/2020/5737289. eCollection 2020.
7
Identification of the Potential Prognostic Markers from the miRNA-lncRNA-mRNA Interactions for Metastatic Renal Cancer via Next-Generation Sequencing and Bioinformatics.通过下一代测序和生物信息学从miRNA-lncRNA-mRNA相互作用中鉴定转移性肾癌的潜在预后标志物
Diagnostics (Basel). 2020 Apr 16;10(4):228. doi: 10.3390/diagnostics10040228.
8
Long Non-Coding RNA LOC648987 Promotes Proliferation and Metastasis of Renal Cell Carcinoma by Regulating Epithelial-Mesenchymal Transition.长链非编码 RNA LOC648987 通过调节上皮-间充质转化促进肾细胞癌的增殖和转移。
Technol Cancer Res Treat. 2021 Jan-Dec;20:1533033821997834. doi: 10.1177/1533033821997834.
9
Overexpression of long non-coding RNA differentiation antagonizing non-protein coding RNA inhibits the proliferation, migration and invasion and promotes apoptosis of renal cell carcinoma.长链非编码 RNA 分化拮抗非编码 RNA 的过表达抑制肾细胞癌的增殖、迁移和侵袭，促进细胞凋亡。
Mol Med Rep. 2017 Oct;16(4):4463-4468. doi: 10.3892/mmr.2017.7135. Epub 2017 Aug 2.
10
lncRNA DUXAP8 inhibits papillary thyroid carcinoma cell apoptosis via sponging the miR‑20b‑5p/SOS1 axis.长链非编码 RNA DUXAP8 通过海绵吸附 miR-20b-5p/SOS1 轴抑制甲状腺乳头状癌细胞凋亡。
Oncol Rep. 2021 May;45(5). doi: 10.3892/or.2021.8015. Epub 2021 Mar 24.

引用本文的文献

1
Long non-coding RNA Knockdown Assisted by CRISPR/Cas9 in Female Cancer Cell Lines Increases Mir-143 .CRISPR/Cas9辅助的长链非编码RNA敲低在女性癌细胞系中可增加Mir-143 。
Iran J Public Health. 2024 Apr;53(4):934-946. doi: 10.18502/ijph.v53i4.15571.
2
Review on Long Non-Coding RNAs as Biomarkers and Potentially Therapeutic Targets for Bacterial Infections.长链非编码RNA作为细菌感染生物标志物和潜在治疗靶点的综述
Curr Issues Mol Biol. 2024 Jul 17;46(7):7558-7576. doi: 10.3390/cimb46070449.
3
LncRNA LINC00847 Accelerates Melanoma Progression by Regulating MiR-133a-3p/TGFBR1 Axis.

本文引用的文献

1
Exploring the mechanisms behind long noncoding RNAs and cancer.探索长链非编码RNA与癌症背后的机制。
Noncoding RNA Res. 2018 Mar 31;3(3):108-117. doi: 10.1016/j.ncrna.2018.03.001. eCollection 2018 Sep.
2
Apoptosis induced by Staphylococcus aureus toxins.金黄色葡萄球菌毒素诱导的细胞凋亡。
Microbiol Res. 2017 Dec;205:19-24. doi: 10.1016/j.micres.2017.08.006. Epub 2017 Aug 18.
3
Renal cell carcinoma.肾细胞癌。
长链非编码 RNA LINC00847 通过调控 miR-133a-3p/TGFBR1 轴促进黑色素瘤进展。
Comb Chem High Throughput Screen. 2024;27(8):1231-1241. doi: 10.2174/1386207326666230816113411.
4
Integrative Analysis Revealed LINC00847 as a Potential Target of Tumor Immunotherapy.整合分析揭示 LINC00847 是肿瘤免疫治疗的潜在靶点。
Appl Biochem Biotechnol. 2023 Oct;195(10):6345-6358. doi: 10.1007/s12010-023-04387-z. Epub 2023 Mar 3.
5
Evaluation of Apoptosis, Cell Proliferation and Cell Cycle Progression by Inactivation of the NEAT1 Long Noncoding RNA in a Renal Carcinoma Cell Line Using CRISPR/Cas9.利用CRISPR/Cas9技术使肾癌细胞系中的NEAT1长链非编码RNA失活，以此评估细胞凋亡、细胞增殖及细胞周期进程
Iran J Biotechnol. 2023 Jan 1;21(1):e3180. doi: 10.30498/ijb.2022.310632.3180. eCollection 2023 Jan.
6
MicroRNA-543 controls pancreatic cancer development by LINC00847-microRNA-543-STK31 axis.微小RNA-543通过LINC00847-微小RNA-543-丝氨酸/苏氨酸激酶31轴控制胰腺癌的发展。
J Gastrointest Oncol. 2022 Dec;13(6):3263-3277. doi: 10.21037/jgo-22-1017.
7
Bidirectional Functional Effects of on Carcinogenesis.[未明确具体物质名称]对致癌作用的双向功能影响。
Microorganisms. 2022 Nov 28;10(12):2353. doi: 10.3390/microorganisms10122353.
8
Construction of prognostic signature of patients with oral squamous cell carcinoma based on pyroptosis-related long non-coding RNAs.基于焦亡相关长链非编码RNA构建口腔鳞状细胞癌患者的预后特征
Front Surg. 2022 Aug 23;9:935765. doi: 10.3389/fsurg.2022.935765. eCollection 2022.
9
Long intergenic non-protein coding RNA 847 promotes laryngeal squamous cell carcinoma progression through the microRNA-181a-5p/zinc finger E-box binding homeobox 2 axis.长链非编码 RNA 847 通过 microRNA-181a-5p/锌指 E 盒结合同源盒 2 轴促进喉鳞状细胞癌进展。
Bioengineered. 2022 Apr;13(4):9987-10000. doi: 10.1080/21655979.2022.2062531.
10
Expression of LINC00847 in Peripheral Blood Mononuclear Cells of Children with Asthma and Its Prediction between Asthma Exacerbation and Remission.外周血单个核细胞中 LINC00847 表达水平对儿童哮喘急性发作与缓解的预测价值
Genet Res (Camb). 2022 Mar 20;2022:5678257. doi: 10.1155/2022/5678257. eCollection 2022.
Nat Rev Dis Primers. 2017 Mar 9;3:17009. doi: 10.1038/nrdp.2017.9.
4
Rapid eradication of colon carcinoma by Clostridium perfringens Enterotoxin suicidal gene therapy.产气荚膜梭菌肠毒素自杀基因疗法快速根除结肠癌
BMC Cancer. 2017 Feb 13;17(1):129. doi: 10.1186/s12885-017-3123-x.
5
Cancer statistics in China, 2015.《中国癌症统计数据 2015》
CA Cancer J Clin. 2016 Mar-Apr;66(2):115-32. doi: 10.3322/caac.21338. Epub 2016 Jan 25.
6
Multi-species sequence comparison reveals conservation of ghrelin gene-derived splice variants encoding a truncated ghrelin peptide.多物种序列比较揭示了编码截短型胃饥饿素肽的胃饥饿素基因衍生剪接变体的保守性。
Endocrine. 2016 Jun;52(3):609-17. doi: 10.1007/s12020-015-0848-7. Epub 2016 Jan 20.
7
Long non-coding RNA chromogenic in situ hybridisation signal pattern correlation with breast tumour pathology.长链非编码RNA显色原位杂交信号模式与乳腺肿瘤病理学的相关性
J Clin Pathol. 2016 Jan;69(1):76-81. doi: 10.1136/jclinpath-2015-203275. Epub 2015 Aug 31.
8
Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer.长链非编码RNA HOTAIR作为一种竞争性内源性RNA，通过吸附胃癌中的miR-331-3p来调节HER2表达。
Mol Cancer. 2014 Apr 28;13:92. doi: 10.1186/1476-4598-13-92.
9
Bacterial programmed cell death: making sense of a paradox.细菌程序性细胞死亡：理解一个悖论。
Nat Rev Microbiol. 2014 Jan;12(1):63-9. doi: 10.1038/nrmicro3136.
10
Dicer is down-regulated in clear cell renal cell carcinoma and in vitro Dicer knockdown enhances malignant phenotype transformation.在透明细胞肾细胞癌中，Dicer表达下调，并且在体外实验中，敲低Dicer可增强恶性表型转化。
Urol Oncol. 2014 Jan;32(1):46.e9-17. doi: 10.1016/j.urolonc.2013.06.011. Epub 2013 Oct 4.