Department of Pediatrics, Ningbo Yinzhou No. 2 Hospital, Ningbo City, Zhejiang Province 315192, China.
Genet Res (Camb). 2022 Mar 20;2022:5678257. doi: 10.1155/2022/5678257. eCollection 2022.
Asthma is defined as a heterogeneous disease that is usually characterized by chronic airway inflammation. Long noncoding RNAs play important roles in various biological processes including inflammation. To know more about the relationships between lncRNAs and asthma, we sought to the role of LINC00847 in peripheral blood mononuclear cells (PBMCs) of children with asthma exacerbation or asthma remission.
Microarray analysis was performed on GSE143192 and GSE165934 datasets to screen differentially expressed lncRNAs (DElncRNAs) in human PBMCs between asthma patients and normal controls. LINC00847 was selected from DElncRNAs in human PBMCs between asthma patients and normal controls for further investigation. The expression levels of LINC00847 were quantified in PBMCs collected from 54 children with asthma exacerbation, 54 children with asthma remission, and 54 healthy children by real-time qPCR. The forced expiratory volume in the first second in percent predicted values (FEV1%), ratio of forced expiratory volume in 1 second to forced vital capacity (FEV1/FVC), and peak expiratory flow rate (PEF%) were tested for evaluation of lung function. The concentration of immunoglobulin E (IgE) and eosinophil count was examined. The serum levels of interleukin-4 (IL-4), interferon- (IFN-), and IL-17A were determined by the ELISA method.
The expression level of LINC00847 in PBMCs of asthma exacerbation children was remarkably higher than that in PBMCs of asthma remission children and healthy children ( < 0.001); the expression level of LINC00847 in PBMCs of asthma remission children was notably higher than that in PBMCs of healthy children ( < 0.001). Pearson correlation analysis revealed that the expression levels of LINC00847 in PBMCs of asthma children were negatively correlated with FEV1% ( = -0.489), FEV1/FVC ( = -0.436), PEF% ( = -0.626), and IFN- level ( = -0.614) of asthma children, but positively correlated with IgE concentration ( = 0.680), eosinophil count ( = 0.780), IL-4 ( = 0.524), and IL-17A ( = 0.622) levels. When LINC00847 expression was used to distinguish asthma exacerbation from asthma remission, a 0.871 AUC (95% CI: 0.805-0.936) was yielded with sensitivity of 79.63% and specificity of 77.78%.
The study demonstrates that increased LINC00847 expression may be associated with the development and progression of asthma, possibly serving as a novel biomarker for predicting asthma exacerbation from asthma remission.
哮喘被定义为一种异质性疾病，通常以慢性气道炎症为特征。长链非编码 RNA 在包括炎症在内的各种生物学过程中发挥重要作用。为了更深入地了解 lncRNA 与哮喘之间的关系，我们研究了 LINC00847 在哮喘发作或缓解的儿童外周血单核细胞 (PBMC) 中的作用。
通过 GSE143192 和 GSE165934 数据集进行微阵列分析，筛选哮喘患者和正常对照人群 PBMC 中差异表达的长链非编码 RNA (DElncRNA)。从哮喘患者和正常对照人群 PBMC 的 DElncRNA 中选择 LINC00847 进行进一步研究。通过实时 qPCR 定量检测 54 例哮喘发作儿童、54 例哮喘缓解儿童和 54 例健康儿童 PBMC 中的 LINC00847 表达水平。通过测定第一秒用力呼气量占预计值的百分比 (FEV1%)、一秒用力呼气量与用力肺活量的比值 (FEV1/FVC)和呼气峰流速 (PEF%)来评估肺功能。检测免疫球蛋白 E (IgE)和嗜酸性粒细胞计数。采用 ELISA 法测定白细胞介素-4 (IL-4)、干扰素- (IFN-)和 IL-17A 的血清水平。
哮喘发作儿童 PBMC 中 LINC00847 的表达水平明显高于哮喘缓解儿童和健康儿童 ( < 0.001)；哮喘缓解儿童 PBMC 中 LINC00847 的表达水平明显高于健康儿童 ( < 0.001)。Pearson 相关分析显示，哮喘患儿 PBMC 中 LINC00847 的表达水平与哮喘患儿的 FEV1% ( = -0.489)、FEV1/FVC ( = -0.436)、PEF% ( = -0.626)和 IFN-水平 ( = -0.614)呈负相关，与 IgE 浓度 ( = 0.680)、嗜酸性粒细胞计数 ( = 0.780)、IL-4 ( = 0.524)和 IL-17A ( = 0.622)水平呈正相关。当使用 LINC00847 表达来区分哮喘发作和哮喘缓解时，AUC 为 0.871(95%CI：0.805-0.936)，灵敏度为 79.63%，特异性为 77.78%。
研究表明，LINC00847 的表达增加可能与哮喘的发生和发展有关，可能成为预测哮喘发作从哮喘缓解的新型生物标志物。
