Pettersson P, Cigolini M, Sjöström L, Smith U, Björntorp P
Acta Med Scand. 1984;215(5):447-51. doi: 10.1111/j.0954-6820.1984.tb17677.x.
Sedimentable cells from human adipose tissue of different origins were cultured under conditions when cell multiplication was prevented but lipid filling optimized (suspension culture). In this way an estimation was obtained of the number of in vivo determined adipose precursor cells (preadipocytes) which were not filled with lipid. Under these conditions no cells from adult, non-obese subjects developed to adipocytes. Cells developing to adipocytes constituted less than 0.02% and 1% of adipocytes in tissues from obese adults and from children, respectively. It was concluded that in vivo determined adipocyte precursor cells (preadipocytes, lipid-free fat cells) are not present in a significant number in human adipose tissue either from adults or from children as tested with the method employed, allowing detection of a large number of such cells in adipose tissue from small rats.
将来自不同来源的人类脂肪组织中的可沉淀细胞，在阻止细胞增殖但优化脂质填充的条件下（悬浮培养）进行培养。通过这种方式，对体内确定的未充满脂质的脂肪前体细胞（前脂肪细胞）数量进行了估计。在这些条件下，来自成年非肥胖受试者的细胞不会发育为脂肪细胞。分别发育为脂肪细胞的细胞在肥胖成年人和儿童组织中的脂肪细胞中所占比例分别不到0.02%和1%。得出的结论是，在所采用的测试方法中，无论是成年人还是儿童的人类脂肪组织中，体内确定的脂肪细胞前体细胞（前脂肪细胞，无脂脂肪细胞）数量都不显著，而该方法能够检测到小大鼠脂肪组织中大量的此类细胞。
