Groeer Saskia, Walther Andreas
A3BMS Lab - Active, Adaptive and Autonomous Bioinspired Material Systems, Institute for Macromolecular Chemistry, University of Freiburg, Stefan-Meier-Straße 31, 79104 Freiburg, Germany.
Nanoscale. 2020 Aug 20;12(32):16995-17004. doi: 10.1039/d0nr04209a.
3D DNA origami provide access to the de novo design of monodisperse and functional bio(organic) nanoparticles, and complement structural protein engineering and inorganic and organic nanoparticle synthesis approaches for the design of self-assembling colloidal systems. We show small 3D DNA origami nanoparticles, which polymerize and depolymerize reversibly to nanotubes of micrometer lengths by applying fuel/antifuel switches. 3D DNA nanocylinders are engineered as a basic building block with different numbers of overhang strands at the open sides to allow for their assembly via fuel strands that bridge both overhangs, resulting in the supracolloidal polymerization. The influence of the multivalent interaction patterns and the length of the bridging fuel strand on efficient polymerization and nanotube length distribution is investigated. The polymerized multivalent nanotubes disassemble through toehold-mediated rehybridization by adding equimolar amounts of antifuel strands. Finally, Förster resonance energy transfer yields in situ insights into the kinetics and reversibility of the nanotube polymerization and depolymerization.
3D DNA折纸技术为单分散功能性生物（有机）纳米颗粒的从头设计提供了途径，并补充了用于自组装胶体系统设计的结构蛋白质工程以及无机和有机纳米颗粒合成方法。我们展示了小型3D DNA折纸纳米颗粒，通过应用燃料/抗燃料开关，它们可以可逆地聚合和解聚成微米长度的纳米管。3D DNA纳米圆柱体被设计为一种基本构建单元，在开口侧具有不同数量的突出链，以便通过连接两个突出链的燃料链进行组装，从而实现超胶体聚合。研究了多价相互作用模式和桥接燃料链长度对高效聚合和纳米管长度分布的影响。通过添加等摩尔量的抗燃料链，聚合的多价纳米管通过链置换介导的再杂交而解体。最后，Förster共振能量转移原位洞察了纳米管聚合和解聚的动力学及可逆性。
