Horticultural Research Institute, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, Shanghai, China.
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
PLoS One. 2020 Aug 11;15(8):e0236980. doi: 10.1371/journal.pone.0236980. eCollection 2020.
Global warming induces heat stress in eggplant, seriously affecting its quality and yield. The response to heat stress is a complex regulatory process; however, the exact mechanism in eggplant is unknown. We analyzed the transcriptome of eggplant under different high-temperature treatments using RNA-Seq technology. Three libraries treated at high temperatures were generated and sequenced. There were 40,733,667, 40,833,852, and 40,301,285 clean reads with 83.98%, 79.69%, and 84.42% of sequences mapped to the eggplant reference genome in groups exposed to 28°C (CK), 38°C (T38), and 43°C (T43), respectively. There were 3,067 and 1,456 DEGs in T38 vs CK and T43 vs CK groups, respectively. In these two DEG groups, 315 and 342 genes were up- and down-regulated, respectively, in common. Differential expression patterns of DEGs in antioxidant enzyme systems, detoxication, phytohormones, and transcription factors under heat stress were investigated. We screened heat stress-related genes for further validation by qRT-PCR. Regulation mechanisms may differ under different temperature treatments, in which heat shock proteins and heat stress transcription factors play vital roles. These results provide insight into the molecular mechanisms of the heat stress response in eggplant and may be useful in crop breeding.
全球变暖使茄子面临热应激，严重影响其品质和产量。对热应激的响应是一个复杂的调控过程；然而，茄子中的确切机制尚不清楚。我们使用 RNA-Seq 技术分析了茄子在不同高温处理下的转录组。生成并测序了三个高温处理的文库。暴露于 28°C（CK）、38°C（T38）和 43°C（T43）的三组分别有 40,733,667、40,833,852 和 40,301,285 条清洁读数，序列分别有 83.98%、79.69%和 84.42%映射到茄子参考基因组。T38 与 CK 相比和 T43 与 CK 相比的差异表达基因（DEGs）分别有 3,067 个和 1,456 个。在这两个 DEG 组中，分别有 315 个和 342 个基因上调和下调，共有 342 个基因上调和下调。研究了抗氧化酶系统、解毒、植物激素和转录因子中 DEGs 在热应激下的差异表达模式。我们通过 qRT-PCR 对热应激相关基因进行了进一步验证筛选。不同温度处理下的调控机制可能不同，其中热休克蛋白和热应激转录因子发挥着重要作用。这些结果为茄子热应激响应的分子机制提供了深入了解，可能对作物育种有用。
