Park Youn Jin, Jang Myoung Jun
Department of Plant Resources, Kongju National University, Yesan Campus, Yesan-eup, Yesan-gun, Chungcheongnam-do 340-702, Korea.
J Fungi (Basel). 2020 Aug 6;6(3):127. doi: 10.3390/jof6030127.
Blue light is an important environmental factor that induces mushroom growth and morphological changes. In this study, after confirming the morphological difference between (LE) under blue light condition (BL) and lightless condition (LL), the increase and decrease in LE protein and the expression of RNA of each protein were confirmed under each condition. LE specimens grown in BL and LL were identified by 253 spots in BL through 2D electrophoresis and LC-MSMS analysis, and 22 types of proteins were identified. It was confirmed that 14 types of proteins showed reduced expression in BL compared to LL. On the other hand, eight kinds of proteins with increased expression in blue light compared to LL were identified. As a result of confirming the difference from the expression pattern in 2D electrophoresis through Quantitative Real-Time PCR, it was confirmed that the expression pattern of the two proteins showed a difference. Therefore, this study will be a key study on the changes in mushroom morphology induced by blue light and the proteins that induce it.
蓝光是诱导蘑菇生长和形态变化的重要环境因素。在本研究中,确认蓝光条件(BL)和无光条件(LL)下(LE)的形态差异后,在每种条件下确认了LE蛋白的增减以及每种蛋白的RNA表达。通过二维电泳和液相色谱 - 串联质谱分析,在BL中鉴定出253个斑点的BL和LL中生长的LE标本,并鉴定出22种蛋白质。证实与LL相比,14种蛋白质在BL中表达降低。另一方面,鉴定出与LL相比在蓝光中表达增加的8种蛋白质。通过定量实时PCR确认与二维电泳中的表达模式的差异,证实两种蛋白质的表达模式存在差异。因此,本研究将成为关于蓝光诱导的蘑菇形态变化及其诱导蛋白的关键研究。