Zhu Yan, Jia Chuanwen, Wang Chao, Zhang Shurui, Yuan Xueyan, Yang Jun, Hsiang Tom, Qi Bao, Peng Zhanwu, Li Yu, Liu Shu-Yan
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun , Jilin Province, 130118, China.
Department of Mycology, College of Plant Protection, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun , Jilin Province, 130118, China.
BMC Genomics. 2024 Dec 24;25(1):1244. doi: 10.1186/s12864-024-11108-1.
Hericium coralloides is a traditional edible and medicinal mushroom. Light is a key factor in forming fruiting bodies of fungi; however, the effects of different light on the yield and morphogenesis of H. coralloides are still unknown. Therefore, the morphology, yield, and transcriptome of H. coralloides under blue, red, and white light conditions were investigated.
Fruiting bodies under blue light exhibited superior morphological traits, such as milky white color, larger size, elongated stalks, and higher spine count, leading to higher yields. Different light treatments led to dramatic transcriptome changes ranging from 10,827 differentially expressed genes (DEGs) induced by blue light in Blue-4d to 11,375 DEGs induced by red light in Red-4d and accounted for 64.56% to 67.81% of all expressed genes. This massive amount of light-responsive genes has never been reported in fungi. Gene Ontology analysis showed that light affected nearly all aspects of life in H. coralloides; suggesting that the influence of light on fungi may have been underestimated. Blue light-induced yield increment may be achieved by specifically upregulating the growth-related processes such as DNA replication, chromosomes, and cell division.
This study offers preliminary insights into the potential role of blue light in modulating gene expression and yield stimulation in H. coralloides, potentially improving cultivation practice.
珊瑚状猴头菇是一种传统的食药用菌。光是真菌子实体形成的关键因素；然而，不同光照对珊瑚状猴头菇产量和形态发生的影响尚不清楚。因此，研究了蓝光、红光和白光条件下珊瑚状猴头菇的形态、产量和转录组。
蓝光下的子实体表现出优异的形态特征，如乳白色、更大的尺寸、更长的菌柄和更高的刺数，从而导致更高的产量。不同的光照处理导致转录组发生显著变化，从蓝光处理4天后诱导的10827个差异表达基因（DEG）到红光处理4天后诱导的11375个DEG，占所有表达基因的64.56%至67.81%。如此大量的光响应基因在真菌中从未有过报道。基因本体分析表明，光几乎影响了珊瑚状猴头菇生活的各个方面；这表明光对真菌的影响可能被低估了。蓝光诱导的产量增加可能是通过特异性上调与生长相关的过程，如DNA复制、染色体和细胞分裂来实现的。
本研究初步揭示了蓝光在调节珊瑚状猴头菇基因表达和提高产量方面的潜在作用，有望改善栽培实践。
