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烟草花叶病毒5' - 前导序列增强翻译能力改变的突变分析。

Mutational analysis of the tobacco mosaic virus 5'-leader for altered ability to enhance translation.

作者信息

Gallie D R, Sleat D E, Watts J W, Turner P C, Wilson T M

机构信息

Department of Virus Research, John Innes Institute, AFRC Institute of Plant Science Research, Norwich, UK.

出版信息

Nucleic Acids Res. 1988 Feb 11;16(3):883-93. doi: 10.1093/nar/16.3.883.

DOI:10.1093/nar/16.3.883
PMID:3278300
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334725/
Abstract

Mutational analysis of the 5'-untranslated leader sequence (omega) of tobacco mosaic virus (TMV) was carried out to determine those sequences necessary for the translational enhancement associated with omega. Five deletion mutants, a single base substitution, and a 25 base replacement mutant were tested for alterations in omega's ability to enhance expression of beta-glucuronidase (GUS) mRNA in tobacco mesophyll protoplasts and Escherichia coli or chloramphenicol acetyltransferase (CAT) mRNA in Xenopus laevis oocytes. Alteration of an eight base subsequence required for the binding of a second ribosome resulted in the loss of translational enhancement in X. laevis oocytes but not in protoplasts. Substantial increases in enhancement were observed for several of the mutants in E. coli.

摘要

对烟草花叶病毒（TMV）5'-非翻译前导序列（ω）进行了突变分析，以确定与ω相关的翻译增强所需的那些序列。测试了五个缺失突变体、一个单碱基替换突变体和一个25碱基替换突变体，以检测ω增强烟草叶肉原生质体中β-葡萄糖醛酸酶（GUS）mRNA表达以及非洲爪蟾卵母细胞中氯霉素乙酰转移酶（CAT）mRNA表达的能力变化。第二个核糖体结合所需的一个八碱基子序列的改变导致非洲爪蟾卵母细胞中翻译增强的丧失，但在原生质体中未丧失。在大肠杆菌中，几个突变体的增强作用显著增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7df5/334725/c96c215ef1c0/nar00145-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7df5/334725/c96c215ef1c0/nar00145-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7df5/334725/c96c215ef1c0/nar00145-0108-a.jpg

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