Department of Cardiology, Aerospace Center Hospital, Peking University Aerospace School of Clinical Medicine, Beijing, China
Department of Cardiology, Aerospace Center Hospital, Peking University Aerospace School of Clinical Medicine, Beijing, China.
J Investig Med. 2020 Oct;68(7):1276-1281. doi: 10.1136/jim-2020-001328. Epub 2020 Aug 11.
This study was performed to determine the effect of ischemic postconditioning on cell apoptosis and angiotensin II receptor type 1 (AT1), connexin 43 (Cx43), and β-tubulin mRNA expression in non-culprit arteries. Non-culprit arterial tissues were isolated from a rabbit myocardial ischemia-reperfusion model and randomly divided into sham, ischemia-reperfusion, and ischemic postconditioning groups. Cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. Expression of angiotensin II, AT1, Cx43, and β-tubulin mRNA was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). TUNEL analysis indicated significantly higher ratios of apoptotic cells in the ischemia-reperfusion group than in the sham group. However, significantly fewer apoptotic cells were observed in the ischemic postconditioning group than in the ischemia-reperfusion group. The qRT-PCR results indicated significantly higher expression of AT1, Cx43, and β-tubulin mRNA in the ischemia-reperfusion group than in the sham group. However, expression of AT1, Cx43, and β-tubulin was lower in the ischemic postconditioning group than in the ischemia-reperfusion group. The ratios of apoptotic cells and mRNA expression of AT1, Cx43, and β-tubulin in non-culprit arteries were increased after ischemia-reperfusion. Ischemic postconditioning may decrease these features and inhibit the progression of non-culprit arteries.
本研究旨在探讨缺血后处理对非罪犯动脉细胞凋亡及血管紧张素 II 受体 1（AT1）、连接蛋白 43（Cx43）和β-微管蛋白 mRNA 表达的影响。取兔心肌缺血再灌注模型非罪犯动脉组织，随机分为假手术组、缺血再灌注组和缺血后处理组。采用末端脱氧核苷酸转移酶 dUTP 缺口末端标记法（TUNEL）检测细胞凋亡。采用实时定量聚合酶链反应（qRT-PCR）检测血管紧张素 II、AT1、Cx43 和β-微管蛋白 mRNA 的表达。TUNEL 分析显示，与假手术组相比，缺血再灌注组细胞凋亡比例显著升高；与缺血再灌注组相比，缺血后处理组细胞凋亡比例显著降低。qRT-PCR 结果显示，与假手术组相比，缺血再灌注组 AT1、Cx43 和β-微管蛋白 mRNA 表达显著升高；与缺血再灌注组相比，缺血后处理组 AT1、Cx43 和β-微管蛋白 mRNA 表达降低。缺血再灌注后非罪犯动脉细胞凋亡比例及 AT1、Cx43 和β-微管蛋白 mRNA 表达增加，缺血后处理可降低这些特征，抑制非罪犯动脉进展。
