通过酶联免疫吸附测定法检测串珠镰刀菌感染玉米中的T-2毒素。
Detection of T-2 toxin in Fusarium sporotrichioides-infected corn by enzyme-linked immunosorbent assay.
作者信息
Gendloff E H, Pestka J J, Swanson S P, Hart L P
出版信息
Appl Environ Microbiol. 1984 May;47(5):1161-3. doi: 10.1128/aem.47.5.1161-1163.1984.
Abstract
A competitive enzyme-linked immunosorbent assay was used to screen for T-2 toxin in Fusarium sporotrichioides -infected corn. The assay detected T-2 toxin in diluted methanol extracts of corn samples at concentrations of 0.05 ng/ml. In infected corn samples, enzyme-linked immunosorbent assay and gas-liquid chromatography estimations of T-2 toxin concentrations were similar.
摘要
采用竞争性酶联免疫吸附测定法筛选串珠镰刀菌感染玉米中的T-2毒素。该测定法能检测到玉米样品稀释甲醇提取物中浓度为0.05纳克/毫升的T-2毒素。在受感染的玉米样品中，酶联免疫吸附测定法和气液色谱法对T-2毒素浓度的估计结果相似。
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