Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P. R. China.
Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, P. R. China.
J Agric Food Chem. 2020 Sep 9;68(36):9690-9696. doi: 10.1021/acs.jafc.0c03181. Epub 2020 Aug 27.
Pumpkins (; Cucurbitaceae) are the rich source of nutrients and valued for their biologically active substances to be used for the treatment of several diseases. The contents, composition, and conformation of starch are the significant quality traits of . Two germplasms were targeted for analysis regarding the taste difference. Results indicated that the total starch contents and amylose/amylopectin ratio were high in CMO-X as compared to CMO-E during each fruit development stage. Scanning electron microscopy and transmission electron microscopy observations revealed that smooth surface starch granules fused together to enhance the starch accumulation. For a comparison of fruit development in CMO-E and CMO-X, the putative pathway for starch metabolism was developed and homologs were identified for each key gene involved in the pathway. and were correlated with the difference in the amylose/amylopectin ratio of CMO-E and CMO-X. Conclusively, the developmental regulation of genes associated with starch accumulation can be considered as an important factor for the determination of fruit quality.
南瓜（葫芦科）是营养丰富的来源，并因其具有生物活性物质而受到重视，可用于治疗多种疾病。淀粉的含量、组成和构象是 的重要质量特征。针对两种种质进行了分析，以了解口感差异。结果表明，在每个果实发育阶段，CMO-X 的总淀粉含量和直链淀粉/支链淀粉比都高于 CMO-E。扫描电子显微镜和透射电子显微镜观察表明，光滑表面的淀粉颗粒融合在一起，以增加淀粉的积累。为了比较 CMO-E 和 CMO-X 中的果实发育，开发了淀粉代谢的假定途径，并鉴定了该途径中每个关键基因的同源物。 和 与 CMO-E 和 CMO-X 的直链淀粉/支链淀粉比差异相关。总之，与淀粉积累相关的基因的发育调控可以被认为是决定果实品质的一个重要因素。
