Broex N J, den Hartog J M, Nouws J F
Tijdschr Diergeneeskd. 1988 Mar 1;113(5):254-9.
The New Netherlands Kidney Test (NNKT) is a method used to detect residues of antibacterial agents in slaughtered animals. This test will shortly be substituted for the current Netherlands Kidney Test (NKT). The new method was tested for its suitability in meat inspection in the field by four district laboratories, comparative studies with the current Netherlands Kidney test being done at the same time. These investigations showed that, when the new test (NNKT) was used, 5.3 per cent of the category of diseased animals and, using the current Netherlands Kidney Test, 2.2 per cent of these animals were positive. When control discs were used, it was shown that reproducibility of the new test (NNKT) within and between the participating laboratories was satisfactory. A method was developed to obtain similar results on re-examination with the NNKT as those obtained in the first inspection. By freezing the duplicate discs at the time of performance of the test and using these discs for re-inspection, reproducible results were obtained with the NNKT. The substance inhibiting the growth of bacteria was identified in a number of specimens diagnosed as positive when the NNKT was used. False-positive results were not recorded in the NNKT.
新荷兰肾脏检测法（NNKT）是一种用于检测屠宰动物体内抗菌剂残留的方法。该检测法不久将取代现行的荷兰肾脏检测法（NKT）。四个地区实验室在实地对这种新方法在肉类检验中的适用性进行了测试，同时与现行的荷兰肾脏检测法进行了对比研究。这些调查表明，使用新检测法（NNKT）时，患病动物类别中有5.3%呈阳性，而使用现行的荷兰肾脏检测法时，这些动物中有2.2%呈阳性。当使用对照圆盘时，结果表明新检测法（NNKT）在参与检测的实验室内部和之间的可重复性令人满意。开发了一种方法，以便在使用NNKT重新检测时获得与首次检测相似的结果。通过在检测时将重复圆盘冷冻，并将这些圆盘用于重新检测，使用NNKT获得了可重复的结果。在一些使用NNKT检测呈阳性的标本中，鉴定出了抑制细菌生长的物质。NNKT未记录到假阳性结果。
