College of Chemical and Biological Engineering, State Key Laboratory of Mining Disaster Prevention and Control Co-founded by Shandong Province and the Ministry of Science and Technology, Shandong University of Science and Technology, Qingdao, 266590, PR China.
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, PR China.
Talanta. 2020 Oct 1;218:121142. doi: 10.1016/j.talanta.2020.121142. Epub 2020 May 23.
N,N-dicarboxymethyl Perylene-diimide (PDI) modified CeCoO nanocomposites were prepared by a two-step method. After modification with PDI molecules, the obtained PDI-CeCoO nanocomposites were demonstrated to possess the heightened peroxidase-like activity, compared with that of pure CeCoO nanoparticles. In the presence of HO, the heightened peroxidase-like behaviors of PDI-CeCoO were evaluated by the oxidation of the colorless substrate 3,3,5,5-tetramethylbenzidine (TMB) into blue oxTMB, which was detected visually only in 4 min. Importantly, a systematic study of catalytic activity of PDI-CeCoO by different means, including fluorescent probe, electrochemical data, diffuse reflection spectra together with free radical scavenger is executed, verifying that the catalytic activity were from O and electron holes (h). And, the transfer of photogenerated carriers in the PDI-CeCoO was the Z-scheme heterojuntion mechanism. Furthermore, the peroxidase-like activity of PDI-CeCoO was significantly inhibited by Glutathione (GSH), resulting in fading of blue oxTMB. Based on this, a colorimetric assay for GSH biosensing has been developed. And, the liner range for GSH detection is from 1 to 10 μM with a detection limit of 0.658 μM. The recovery of GSH with different concentrations from 90.0% to 105.9% and the relative standard deviation (RSD) from 1.9% to 5.1%. This colorimetric sensor can be used to detect GSH in real samples.
N,N-二羧甲基苝二酰亚胺（PDI）修饰的 CeCoO 纳米复合材料通过两步法制备。在 PDI 分子修饰后，与纯 CeCoO 纳米粒子相比，所得的 PDI-CeCoO 纳米复合材料被证明具有增强的过氧化物酶样活性。在 HO 的存在下，通过将无色底物 3,3,5,5-四甲基联苯胺（TMB）氧化为蓝色 oxTMB 来评估 PDI-CeCoO 的增强过氧化物酶样行为，仅在 4 分钟内即可通过肉眼观察到。重要的是，通过荧光探针、电化学数据、漫反射光谱和自由基清除剂等不同手段对 PDI-CeCoO 的催化活性进行了系统研究，验证了催化活性来自 O 和电子空穴（h）。并且，PDI-CeCoO 中光生载流子的转移是 Z 型异质结机制。此外，PDI-CeCoO 的过氧化物酶样活性被谷胱甘肽（GSH）显著抑制，导致蓝色 oxTMB 褪色。基于此，开发了用于 GSH 生物传感的比色测定法。并且，GSH 检测的线性范围为 1 至 10 μM，检测限为 0.658 μM。从 90.0%到 105.9%的不同浓度的 GSH 回收率和相对标准偏差（RSD）从 1.9%到 5.1%。这种比色传感器可用于检测真实样品中的 GSH。
