College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao, 266590, People's Republic of China.
Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People's Republic of China.
Mikrochim Acta. 2023 Jun 21;190(7):270. doi: 10.1007/s00604-023-05846-4.
The peroxidase-like activity of CdVO nanorods has been considerably improved by modification with N, N-dicarboxymethyl perylene-diimide (PDI) as a photosensitizer. The peroxidase-like behaviors are evaluated by virtue of the colorless chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB), which is fast changed into blue oxTMB in the presence of HO in only 90 s. PDI-CdVO exhibits high stability at elevated temperatures and PDI-CdVO retains more than 70% of its catalytic activity over a wide range of 15 to 60 °C. The catalytic mechanism of PDI-CdVO is ascribed to the synergistic interaction between PDI and CdVO and the generation of •O radicals. Based on the enhanced peroxidase-like activity of PDI-CdVO, a selective colorimetric sensor has been constructed for HO and pyrogallol (PG) with detection limits of 36.5 μM and 0.179 μM, respectively. The feasibility of the proposed sensing platform has been validated by detecting HO in milk and pyrogallol in tap water.
CdVO 纳米棒的过氧化物酶样活性通过用 N，N-二羧甲基苝二酰亚胺（PDI）作为光敏剂进行修饰得到了显著提高。过氧化物酶样行为通过无色显色底物 3,3',5,5'-四甲基联苯胺（TMB）进行评估，在存在 HO 的情况下，仅在 90 秒内将 TMB 快速转化为蓝色 oxTMB。PDI-CdVO 在高温下具有高稳定性，并且在 15 至 60°C 的宽范围内保留超过 70%的催化活性。PDI-CdVO 的催化机制归因于 PDI 和 CdVO 之间的协同相互作用以及 •O 自由基的生成。基于 PDI-CdVO 增强的过氧化物酶样活性，构建了一种用于检测 HO 和邻苯三酚（PG）的选择性比色传感器，其检测限分别为 36.5μM 和 0.179μM。通过检测牛奶中的 HO 和自来水中的邻苯三酚，验证了所提出的传感平台的可行性。
