Yan Shoucai, Guo Hongda, Tan Jingjing, Jiang Jianlan, Liang Jingjing, Yan Shuyu, Xiao Haibo
Department of Chemistry, Shanghai Normal University, Shanghai, 200234, PR China.
Department of Chemistry, Shanghai Normal University, Shanghai, 200234, PR China.
Talanta. 2020 Oct 1;218:121210. doi: 10.1016/j.talanta.2020.121210. Epub 2020 May 30.
Two spirobifluorene-based two-photon fluorescent probes for the detection of hydrazine, namely SPF-MN and SPF- PA, have been designed and synthesized. Along with the addition of hydrazine to a solution of SPF-MN, both a colorimetric change from yellow to colorless and a fluorescence change from yellow to blue (Under 365 nm UV light) can be observed by ''naked-eye''. Probe SPF- PA displayed response toward hydrazine with fluorescence enhancement. The detection limits are 6.9 μM for SPF- PA and 0.29 μM for SPF-MN, respectively. Moreover, SPF-MN and SPF- PA can be used as two-photon fluorescent probes for hydrazine with large two-photon absorption cross-sections and used for the imaging of hydrazine in living cells. Specially, SPF-PA can located at the surface of the cells, and it is the first fluorescent probe which possesses the capability of sensing intercellular hydrazine. Besides, SPF-MN is the first colorimetric two-photon fluorescent probe for meeting the criteria of both hydrazine bioimaging and visual detection of hydrazine in solution.
已设计并合成了两种用于检测肼的基于螺二芴的双光子荧光探针，即SPF-MN和SPF-PA。随着肼加入到SPF-MN溶液中，肉眼可观察到颜色从黄色变为无色以及荧光从黄色变为蓝色（在365nm紫外光下）。探针SPF-PA对肼表现出荧光增强响应。SPF-PA和SPF-MN的检测限分别为6.9μM和0.29μM。此外，SPF-MN和SPF-PA可作为具有大二光子吸收截面的肼双光子荧光探针，并用于活细胞中肼的成像。特别地，SPF-PA可定位在细胞表面，它是首个具有检测细胞间肼能力的荧光探针。此外，SPF-MN是首个满足肼生物成像和溶液中肼视觉检测标准的比色双光子荧光探针。
