National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai 201306, China; Hunan Engineering Research Center for Utilization of Characteristics of Aquatic Resources, Hunan Agricultural University, Changsha 410128, China.
National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai 201306, China.
Environ Toxicol Pharmacol. 2020 Nov;80:103466. doi: 10.1016/j.etap.2020.103466. Epub 2020 Aug 14.
Praziquantel (PZQ) is a drug commonly used to treat some parasitic infections in animals. This study aimed to apply a reliable and simple method to identify important biological metabolites relevant to PZQ in crucian carp (Carassius auratus) to decipher the metabolic pathways and provide a basis for developing new anti-parasite drugs. The experimental group of crucian carp was administered oral PZQ at a dose of 10 mg kg via a stomach feed tube. All biological blood samples were analysed using liquid chromatography electrospray ionization/quadrupole time-of-flight mass spectrometry (LC ESI/Q-TOF MS). MetPA analysis was used to identify relevant pathways for PZQ in crucian carp. Thirty-five potential metabolic pathways were revealed by MetPA network software. Furthermore, the chemical structures of the related metabolites and pathways were identified by comparison with data obtained from free online databases. Forty-four significantly differentially abundant endogenous metabolites were found in the PZQ-treated crucian carp. The changes in metabolomic profiles and pathways induced by PZQ played a role in inhibiting pathogenesis.
吡喹酮（PZQ）是一种常用于治疗动物寄生虫感染的药物。本研究旨在应用可靠且简单的方法来鉴定鲫鱼（Carassius auratus）中与 PZQ 相关的重要生物代谢物，以揭示其代谢途径，并为开发新的抗寄生虫药物提供依据。实验组鲫鱼通过胃饲管口服给予 10mg/kg 的 PZQ。使用液相色谱电喷雾电离/四极杆飞行时间质谱（LC ESI/Q-TOF MS）分析所有生物血液样本。使用 MetPA 分析鉴定鲫鱼中 PZQ 的相关途径。MetPA 网络软件揭示了 35 个潜在的代谢途径。此外，通过与从免费在线数据库中获得的数据进行比较，鉴定了相关代谢物和途径的化学结构。在 PZQ 处理的鲫鱼中发现了 44 种显著差异丰度的内源性代谢物。PZQ 诱导的代谢组学图谱和途径的变化在抑制发病机制中发挥作用。
