Al-Saeed Adel Talib, Abdo Jassim Mohammed, Gorgess Rouad Ganim
Department of Microbiology, College of Medicine, Duhok Unuversity, Duhok, Iraq.
Department of Microbiology, College of Pharmacy, Duhok University, Duhok, Iraq.
J Pak Med Assoc. 2020 Jul;70(7):1251-1255. doi: 10.5455/JPMA.21273.
This is the first study to detect cryptosporidiosis among children in Duhok city/Kurdistan region of Iraq. The study included 332 stool samples of children using Modified Ziehl Neelsen Method (MZNM) and 122 of these were randomly selected to detect Cryptosporidium by ELISA. By MZNM, the infection rate of Cryptosporidium oocysts was 66.95% in children; 44.68% in immunocompromised (ICD) and 22.27% in immunocompetent (ICT) group. Among ICT children, the infection rate was highest among less than one year of age (39.34%) in diarrheoic group, while in non-diarrheoic group, it was highest among 1-4 years age (28.57%). Among ICD children, the relations were non-significant among ages. Out of 122 stool samples of children, 26 (21.31%), and 30 (24.59%) were positive by MZNM and ELISA, respectively. In conclusion, ELISA was more sensitive and specific than MZNM which were 82.5% and 90.91% respectively. This study indicates that asymptomatic infection is common among different age groups of children.
这是在伊拉克库尔德斯坦地区杜胡克市儿童中检测隐孢子虫病的第一项研究。该研究使用改良齐-尼氏法（MZNM）对332名儿童的粪便样本进行检测，并随机选取其中122份样本通过酶联免疫吸附测定（ELISA）检测隐孢子虫。通过MZNM检测，儿童中隐孢子虫卵囊感染率为66.95%；免疫功能低下（ICD）组为44.68%，免疫功能正常（ICT）组为22.27%。在ICT儿童中，腹泻组1岁以下儿童感染率最高（39.34%），而非腹泻组1 - 4岁儿童感染率最高（28.57%）。在ICD儿童中，各年龄组之间的关系不显著。在122份儿童粪便样本中，通过MZNM和ELISA检测分别有26份（21.31%）和30份（24.59%）呈阳性。总之，ELISA比MZNM更敏感和特异，其敏感性和特异性分别为82.5%和90.91%。本研究表明无症状感染在不同年龄组儿童中很常见。
