Yang Huanhuan, Shen Fengyi, Wang Hexuan, Zhao Tingting, Zhang He, Jiang Jingbin, Xu Xiangyang, Li Jingfu
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Mucai Street 59, Xiangfang District, Harbin, 150030, China.
BMC Plant Biol. 2020 Aug 15;20(1):376. doi: 10.1186/s12870-020-02588-w.
Tomato gray leaf spot caused by Stemphylium lycopersici (S. lycopersici) is a serious disease that can severely hinder tomato production. To date, only Sm has been reported to provide resistance against this disease, and the molecular mechanism underlying resistance to this disease in tomato remains unclear. To better understand the mechanism of tomato resistance to S. lycopersici, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR)-based analysis, physiological indexes, microscopy observations and transgenic technology were used in this study.
Our results showed that the expression of SlERF01 was strongly induced by S. lycopersici and by exogenous applications of the hormones salicylic acid (SA) and jasmonic acid (JA). Furthermore, overexpression of SlERF01 enhanced the hypersensitive response (HR) to S. lycopersici and elevated the expression of defense genes in tomato. Furthermore, the accumulation of lignin, callose and hydrogen peroxide (HO) increased in the transgenic lines after inoculation with S. lycopersici. Taken together, our results showed that SlERF01 played an indispensable role in multiple SA, JA and reactive oxygen species (ROS) signaling pathways to provide resistance to S. lycopersici invasion. Our findings also indicated that SlERF01 could activate the expression of the PR1 gene and enhance resistance to S. lycopersici.
We identified the SlERF01 gene, which encodes a novel tomato AP2/ERF transcription factor (TF). Functional analysis revealed that SlERF01 positively regulates tomato resistance to S. lycopersici. Our findings indicate that SlERF01 plays a key role in multiple SA, JA and ROS signaling pathways to provide resistance to invasion by S. lycopersici. The findings of this study not only help to better understand the mechanisms of response to pathogens but also enable targeted breeding strategies for tomato resistance to S. lycopersici.
由番茄匍柄霉（Stemphylium lycopersici，S. lycopersici）引起的番茄灰叶斑病是一种严重病害，会严重阻碍番茄生产。迄今为止，仅有Sm被报道对该病具有抗性，而番茄对这种病害的抗性分子机制仍不清楚。为了更好地理解番茄对S. lycopersici的抗性机制，本研究采用了基于实时定量逆转录-聚合酶链反应（qRT-PCR）的分析、生理指标、显微镜观察和转基因技术。
我们的结果表明，SlERF01的表达受到S. lycopersici以及外源施加水杨酸（SA）和茉莉酸（JA）的强烈诱导。此外，SlERF01的过表达增强了番茄对S. lycopersici的过敏反应（HR），并提高了防御基因在番茄中的表达。此外，接种S. lycopersici后，转基因株系中木质素、胼胝质和过氧化氢（H₂O₂）的积累增加。综上所述，我们的结果表明，SlERF01在多种SA、JA和活性氧（ROS）信号通路中发挥不可或缺的作用，以提供对S. lycopersici入侵的抗性。我们的研究结果还表明，SlERF01可以激活PR1基因的表达并增强对S. lycopersici的抗性。
我们鉴定出SlERF01基因，其编码一种新型番茄AP2/ERF转录因子（TF）。功能分析表明，SlERF01正向调控番茄对S. lycopersici的抗性。我们的研究结果表明，SlERF01在多种SA、JA和ROS信号通路中发挥关键作用，以提供对S. lycopersici入侵的抗性。本研究结果不仅有助于更好地理解对病原体的反应机制，还能为番茄抗S. lycopersici的定向育种策略提供依据。
