清酒乳杆菌抑制胶原诱导性关节炎，并调节辅助性 T 细胞 17 和调节性 B 细胞的分化。

Lactobacillus sakei suppresses collagen-induced arthritis and modulates the differentiation of T helper 17 cells and regulatory B cells.

机构信息

Rheumatism Research Center, Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, Seoul, 06591, South Korea.

Laboratory of Immune Network, Catholic Research Institute of Medical Science, College of Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.

出版信息

J Transl Med. 2020 Aug 15;18(1):317. doi: 10.1186/s12967-020-02477-8.

BACKGROUND

To evaluate the immunomodulatory effect of Lactobacillus sakei in a mouse model of rheumatoid arthritis (RA) and in human immune cells.

METHODS

We evaluated whether L. sakei reduced the severity of collagen-induced arthritis (CIA) and modulated interleukin (IL)-17 and IL-10 levels, as well as whether it affected the differentiation of CD4 T cells and regulatory B cells. We evaluated osteoclastogenesis after culturing bone marrow-derived mononuclear cells with L. sakei.

RESULTS

The differentiation of T helper 17 cells and the serum level of IL-17 were suppressed by L. sakei in both human peripheral blood mononuclear cells and mouse splenocytes. The serum level of IL-10 was significantly increased in the L. sakei-treated group, whereas the regulatory T cell population was unchanged. The population of regulatory B cells significantly increased the in L. sakei-treated group. Oral administration of L. sakei reduced the arthritis incidence and score in mice with CIA. Finally, osteoclastogenesis and the mRNA levels of osteoclast-related genes were suppressed in the L. sakei-treated group.

CONCLUSION

L. sakei exerted an anti-inflammatory effect in an animal model of RA, regulated Th17 and regulatory B cell differentiation, and suppressed osteoclastogenesis. Our findings suggest that L. sakei has therapeutic potential for RA.

背景

评估清酒乳杆菌在类风湿关节炎（RA）小鼠模型和人免疫细胞中的免疫调节作用。

方法

我们评估了清酒乳杆菌是否能减轻胶原诱导性关节炎（CIA）的严重程度，并调节白细胞介素（IL）-17 和 IL-10 水平，以及它是否影响 CD4 T 细胞和调节性 B 细胞的分化。我们评估了培养骨髓来源的单核细胞后清酒乳杆菌对破骨细胞生成的影响。

结果

清酒乳杆菌在人外周血单核细胞和小鼠脾细胞中均抑制辅助性 T 细胞 17 细胞的分化和血清中 IL-17 的水平。清酒乳杆菌治疗组血清中 IL-10 水平显著升高，而调节性 T 细胞群体不变。清酒乳杆菌治疗组调节性 B 细胞群体显著增加。口服清酒乳杆菌可降低 CIA 小鼠的关节炎发生率和评分。最后，清酒乳杆菌治疗组破骨细胞生成和破骨细胞相关基因的 mRNA 水平受到抑制。