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C杂草(L.)中铝的过量积累及相关抗氧化反应

Aluminium accumulation in excess and related anti-oxidation responses in C weed ( L.).

作者信息

Sarkar Bipul, Saha Indraneel, De Arnab Kumar, Ghosh Arijit, Adak M K

机构信息

Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Kalyani, West Bengal 741235 India.

Department of Botany, Karimpur Pannadevi College, Karimpur, West Bengal 741152 India.

出版信息

Physiol Mol Biol Plants. 2020 Aug;26(8):1583-1598. doi: 10.1007/s12298-020-00840-z. Epub 2020 Jul 15.

Abstract

C species, L. exhibited a significant bioaccumulation of aluminium (Al) through the duration of 3- and 5-days exposure. As compared to control, appeared as excess-accumulator with maximum 5.85-fold bioaccumulation of Al in root. Cellular responses to Al tolerance initially scored tissue specific distribution of metal through cortical layers revealed by electron microscopy. The affected cells changed an oxidative status as read by histochemical stains, particularly, for hydrogen peroxide. Osmotic stress and its stability were scored by maximum proline and free amino acids accumulation with 1.53 and 1.59-fold increase over control. The accumulation of phenolics and flavonoids were over expressed in the ranges of 2.48-2.50-fold and 2.00-1.5-fold at 3- and 5-days respectively against control. Anti-oxidation to detoxify Al stress was facilitated by variants of peroxidases. For exclusion mechanism of metal, esterase activity significantly over expressed with maximum value of 1.80-fold at 5-days. The polymorphism of esterase exhibited few significant over produced bands, varied in numbers as detected by densitometric scanning. Moreover, plant extract was satisfactorily potential under in vitro anti-oxidation systems through assay of 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), ferric chelation activity etc. Therefore, weeds like would be a bioprospecting in role likely involved in phytoremediation of metal.

摘要

在3天和5天的暴露期内,C物种L.表现出显著的铝(Al)生物累积。与对照组相比,其在根部的铝生物累积量最高可达5.85倍,表现为超累积植物。通过电子显微镜观察发现,对铝耐受性的细胞反应最初表现为金属在皮层组织中的特异性分布。受影响的细胞通过组织化学染色显示出氧化状态的变化,特别是过氧化氢。通过脯氨酸和游离氨基酸的最大积累来评估渗透胁迫及其稳定性,与对照组相比分别增加了1.53倍和1.59倍。酚类和黄酮类化合物的积累在3天和5天时分别比对照组高出2.48 - 2.50倍和2.00 - 1.5倍。过氧化物酶变体促进了对铝胁迫的抗氧化解毒作用。对于金属的排除机制,酯酶活性在5天时显著过表达,最大值为1.80倍。酯酶的多态性表现出一些显著的过表达条带,通过密度扫描检测其数量不同。此外,通过1,1 - 二苯基 - 2 - 苦基肼(DPPH)、2,2'-联氮 - 双 - 3 - 乙基苯并噻唑啉 - 6 - 磺酸(ABTS)、铁螯合活性等测定,植物提取物在体外抗氧化系统中具有令人满意的潜力。因此,像这样的杂草可能在植物修复金属方面发挥生物勘探作用。

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