使用胺脱氢酶变体对消旋伯胺进行动力学拆分
Kinetic Resolution of Racemic Primary Amines Using Amine Dehydrogenase Variant.
作者信息
Tseliou Vasilis, Knaus Tanja, Vilím Jan, Masman Marcelo F, Mutti Francesco G
机构信息
van 't Hoff Institute for Molecular Sciences HIMS-Biocat University of Amsterdam Science Park 904 1098 XH Amsterdam (The Netherlands.
出版信息
ChemCatChem. 2020 Apr 20;12(8):2184-2188. doi: 10.1002/cctc.201902085. Epub 2020 Mar 6.
A NADH-dependent engineered amine dehydrogenase from (LE-AmDH-v1) was applied together with a NADH-oxidase from (NOx) for the kinetic resolution of pharmaceutically relevant racemic α-chiral primary amines. The reaction conditions (e. g., pH, temperature, type of buffer) were optimised to yield -configured amines with up to >99 % .
来自[具体来源1]的一种依赖烟酰胺腺嘌呤二核苷酸（NADH）的工程化胺脱氢酶（LE-AmDH-v1）与来自[具体来源2]的烟酰胺腺嘌呤二核苷酸氧化酶（NOx）一起用于动力学拆分与药物相关的外消旋α-手性伯胺。对反应条件（例如pH值、温度、缓冲液类型）进行了优化，以生成对映体过量值高达>99%的[具体构型]构型胺。
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