An Update: Enzymatic Synthesis for Industrial Applications.

Supported by rapid technological advancements, biocatalytic applications have matured into sustainable, scalable, and cost-competitive alternatives to established chemical catalysis. This review presents the most recent examples of enzyme-based solutions for the manufacturing of molecules with extended carbon-carbon frameworks and multiple stereogenic centers at commercial scale, including peptide building blocks, (rare) sugars, synthetic (oligo)nucleotides, and terpenoids, such as (-)-Ambrox. Novel enzyme classes are highlighted along with their potential applications-the synthesis of DNA/RNA, the depolymerization of synthetic plastics, or fully enzymatic protection/deprotection schemes-pointing toward the diversification and broader industrial utilization of biocatalysis-based processes.