Wang Gangzheng, Li Min, Zhang Chenghua, Cheng Huijiao, Gao Yu, Deng Wangqiu, Li Taihui
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
College of Agriculture and Animal Husbandry, Tibet University, Nyingchi, 860000 Tibet, China.
Comput Struct Biotechnol J. 2020 Jul 25;18:2081-2094. doi: 10.1016/j.csbj.2020.07.014. eCollection 2020.
has a similar metabolite profile to , a highly regarded fungus used for traditional Chinese medicine with high nutritional and medicinal value. Although the genome sequence of has been reported, relatively little is known about the regulatory networks for fruiting body development and about the metabolite biosynthesis pathways. In order to address this, an analysis of transcriptome and proteome at differential developmental stages of was performed. In total, 9076 genes were found to be expressed and 2040 proteins were identified. There were a large number of genes that were significantly differentially expressed between the mycelial stage and the stages. Interestingly, the correlation between the transcriptomic and proteomic data was low, suggesting the importance of the post-transcriptional processes in the growth and development of . Among the genes/proteins that were both differentially expressed during the developmental process, there were numerous heat shock proteins and transcription factors. In addition, there were numerous proteins involved in terpenoid, ergosterol, adenosine and polysaccharide biosynthesis that also showed significant downregulation in their expression levels during the developmental process. Furthermore, both tryptophan and tryptamine were present at higher levels in the primordium stage. However, indole-3-acetic acid (IAA) levels continuously decreased as development proceeded, and the enzymes involved in IAA biosynthesis were also clearly differentially downregulated. These data could be meaningful in studying the molecular mechanisms of fungal development, and for the industrial and medicinal application of macro-fungi.
具有与 相似的代谢物谱， 是一种备受推崇的用于传统中药的真菌，具有很高的营养和药用价值。尽管 的基因组序列已被报道，但关于子实体发育的调控网络以及代谢物生物合成途径却知之甚少。为了解决这个问题，对 不同发育阶段的转录组和蛋白质组进行了分析。总共发现9076个基因表达，鉴定出2040种蛋白质。在菌丝体阶段和其他阶段之间有大量基因存在显著差异表达。有趣的是，转录组数据和蛋白质组数据之间的相关性较低，这表明转录后过程在 的生长和发育中具有重要性。在发育过程中差异表达的基因/蛋白质中，有许多热休克蛋白和转录因子。此外，有许多参与萜类、麦角固醇、腺苷和多糖生物合成的蛋白质在发育过程中其表达水平也显著下调。此外，色氨酸和色胺在原基阶段含量较高。然而，随着发育的进行，吲哚-3-乙酸（IAA）水平持续下降，参与IAA生物合成的酶也明显差异下调。这些数据对于研究真菌发育的分子机制以及大型真菌的工业和药用应用可能具有重要意义。
