State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China; College of Agriculture and Animal Husbandry, Tibet University, Nyingchi 860000, Tibet, China.
Gene. 2020 Jun 15;743:144563. doi: 10.1016/j.gene.2020.144563. Epub 2020 Mar 10.
DnaJ is an important molecular chaperone, with significant roles in growth, development, and stress resistance. Studies on the DnaJ gene family in macro-fungi such as Cordyceps spp. s.l. is scare. In this study, 22, 20, and 24 putative DnaJ genes were identified in Tolypocladium guangdongense, Ophiocordyceps sinensis, and C. militaris, respectively. They were classified into four groups based on the presence of the J, zinc finger, and C-terminal domains. We mainly studied the T. guangdongense DnaJ genes being located in the endoplasmic reticulum, cytoplasm, mitochondrion, and nucleus. Phylogenetic analysis revealed gene duplications during the evolutionary process. Multiple cis-elements and transcription factor binding sites were observed in the promoter, suggesting their involvement in the response to multiple stresses. qRT-PCR analysis showed that 63.63% and 45.45% of T. guangdongense DnaJ genes were differentially expressed under cold and heat stress, respectively, indicating their involvement in the response to temperature stress. Many T. guangdongense DnaJ genes in the primordium and fruiting body exhibited differential expression, in comparison to those in the mycelium, suggesting a regulatory role in its growth and development process. These findings will facilitate further functional analysis, and provide information on the classification and conservative functions of DnaJ proteins in macro-fungi.
DnaJ 是一种重要的分子伴侣，在生长、发育和抗应激方面发挥着重要作用。对蛹虫草属等大型真菌中的 DnaJ 基因家族的研究较少。本研究分别在广东虫草、冬虫夏草和蛹虫草中鉴定了 22、20 和 24 个假定的 DnaJ 基因。根据 J、锌指和 C 端结构域的存在，它们被分为四个组。我们主要研究了广东虫草的 DnaJ 基因，这些基因定位于内质网、细胞质、线粒体和细胞核中。系统发育分析表明，在进化过程中发生了基因复制。启动子中观察到多个顺式元件和转录因子结合位点，表明它们参与了对多种胁迫的反应。qRT-PCR 分析表明，广东虫草的 63.63%和 45.45%的 DnaJ 基因在冷和热胁迫下差异表达，表明它们参与了对温度胁迫的反应。与菌丝体相比，原基和子实体中的许多广东虫草 DnaJ 基因表达差异，表明它们在生长和发育过程中起调控作用。这些发现将有助于进一步的功能分析，并提供有关 DnaJ 蛋白在大型真菌中的分类和保守功能的信息。
