The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, UK.
Peter Medawar Building for Pathogen Research and Oxford NIHR Biomedical Research Centre, Nuffield Department of Medicine, University of Oxford, South Parks Road, Oxford OX1 3SY, UK.
J Immunol Res. 2020 Aug 6;2020:8624963. doi: 10.1155/2020/8624963. eCollection 2020.
Single-cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited-volume samples, advancing prospects of a new era of systems immunology. The power of single-cell RNA sequencing offers various opportunities to decipher the immune response to infectious diseases and vaccines. Here, we describe the potential uses of single-cell RNA sequencing methods in prophylactic vaccine development, concentrating on infectious diseases including COVID-19. Using examples from several diseases, we review how single-cell RNA sequencing has been used to evaluate the immunological response to different vaccine platforms and regimens. By highlighting published and unpublished single-cell RNA sequencing studies relevant to vaccinology, we discuss some general considerations how the field could be enriched with the widespread adoption of this technology.
单细胞 RNA 测序允许从有限体积的样本中对细胞免疫反应进行高度详细的分析，为系统免疫学的新时代带来了广阔的前景。单细胞 RNA 测序的强大功能为解析传染病和疫苗的免疫反应提供了各种机会。在这里，我们描述了单细胞 RNA 测序方法在预防性疫苗开发中的潜在用途，重点关注包括 COVID-19 在内的传染病。我们通过几个疾病的例子来回顾单细胞 RNA 测序如何用于评估不同疫苗平台和方案的免疫反应。通过强调与疫苗学相关的已发表和未发表的单细胞 RNA 测序研究，我们讨论了如何通过广泛采用这项技术来丰富该领域的一些一般性考虑。
