Yen Hung-Chi, Kuo Tsung-Rong, Huang Min-Hui, Huang Hao-Kang, Chen Chia-Chun
Department of Chemistry, National Taiwan Normal University, Taipei 116, Taiwan.
Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110, Taiwan.
ACS Omega. 2020 Jul 28;5(31):19771-19777. doi: 10.1021/acsomega.0c02533. eCollection 2020 Aug 11.
High-throughput and rapid arsenite (As(III)) monitoring is an urgent task to deal with the critical threat from As(III) contamination in the environment. In this study, an effective, portable, and sensitive As(III) assay was developed using the plasmonic silver (pAg) chips for As(III) detection. The pAg chips were fabricated by a simple seed-mediated method to grow the silver nanoisland films (Ag-NIFs) with the compact nanoislands and adjustable interisland gaps on the large-sized substrates. With appropriate surface functionalization and optimal chip manufacturing, Cy7.5 fluorescence dye can be immobilized on the surface of Ag-NIFs in the presence of As(III) to output the enhanced fluorescence signals up to 10-fold and improve the detection limit of As(III) less than 10 ppb. According to our results, the high-throughput detection measurements and wide dynamic range over 4 orders of magnitude implied the broad prospects of pAg chips in fluorescence-enhanced assays. The proposed As(III) assay has shown great opportunities for the practical application of ultratrace As(III) monitoring.
高通量快速监测砷（III）是应对环境中砷（III）污染带来的严峻威胁的一项紧迫任务。在本研究中，开发了一种有效、便携且灵敏的砷（III）检测方法，使用等离子体银（pAg）芯片进行砷（III）检测。通过一种简单的种子介导方法制备pAg芯片，以在大尺寸基底上生长具有紧密纳米岛和可调节岛间间隙的银纳米岛薄膜（Ag-NIFs）。经过适当的表面功能化和优化芯片制造，在砷（III）存在的情况下，Cy7.5荧光染料可固定在Ag-NIFs表面，输出高达10倍的增强荧光信号，并将砷（III）的检测限提高到小于10 ppb。根据我们的结果，高通量检测测量以及超过4个数量级的宽动态范围暗示了pAg芯片在荧光增强检测中的广阔前景。所提出的砷（III）检测方法在超痕量砷（III）监测的实际应用中显示出巨大潜力。
