PG and Research Department of Biotechnology, Mahendra Arts and Science College (Autonomous), Kalippatti, Namakkal, Tamil Nadu, 637 501, India.
Food Chemistry and Molecular Cancer Biology Laboratory, Department of Animal Health and Management, Alagappa University, Karaikudi, Tamil Nadu, 630 003, India.
Bioprocess Biosyst Eng. 2021 Feb;44(2):217-223. doi: 10.1007/s00449-020-02426-1. Epub 2020 Aug 16.
The present investigation reports a simple, rapid, inexpensive, and eco-friendly approach for synthesizing Amphiroa rigida-mediated silver nanoparticles (AR-AgNPs) for the first time. The biomimetic synthesized AR-AgNPs were characterized by both spectral and microscopic analysis. The UV-visible spectrum showed the surface plasmon peak at 420 nm, which indicated the formation of AR-AgNPs. X-ray diffraction characterization of AR-AgNPs showed a face-centered cubic crystal (25 nm) and the transmission electron microscope micrograph showed spherical shape. The functional group of polysaccharide that plays a major role as a stabilizing and reducing agent is confirmed by Fourier-transform infrared spectroscopy. The biomimetic synthesized AR-AgNPs showed antibacterial activity against Staphylococcus aureus (21 ± 0.2 mm) and Pseudomonas aeruginosa (15 ± 0.2 mm). Further, the cytotoxic effects of AR-AgNPs against MCF-7 human breast cancer cells were observed through acridine orange-ethidium bromide and Hoechst staining. Besides, AR-AgNPs are found to be inhibit the growth of 3rd and 4th instar larvae of Aedes aegypti in a dose-dependent manner.
本研究首次报道了一种简单、快速、廉价且环保的方法，用于合成 Amphiroa rigida 介导的银纳米粒子 (AR-AgNPs)。仿生合成的 AR-AgNPs 通过光谱和显微镜分析进行了表征。紫外-可见光谱在 420nm 处显示出表面等离子体峰，表明形成了 AR-AgNPs。AR-AgNPs 的 X 射线衍射特征显示出面心立方晶体（25nm），透射电子显微镜照片显示出球形。傅里叶变换红外光谱证实了多糖作为主要稳定剂和还原剂的官能团。仿生合成的 AR-AgNPs 对金黄色葡萄球菌（21±0.2mm）和铜绿假单胞菌（15±0.2mm）表现出抗菌活性。此外，通过吖啶橙-溴化乙锭和 Hoechst 染色观察到 AR-AgNPs 对 MCF-7 人乳腺癌细胞的细胞毒性作用。此外，AR-AgNPs 被发现以剂量依赖的方式抑制埃及伊蚊 3 龄和 4 龄幼虫的生长。
