Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Kamýcká 129, Prague 6 - Suchdol, 165 00, Czechia.
Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy.
Int J Syst Evol Microbiol. 2020 Sep;70(9):5040-5047. doi: 10.1099/ijsem.0.004378.
A fructose-6-phosphate phosphoketolase-positive strain (GSD1FS) was isolated from a faecal sample of a 3 weeks old German Shepherd dog. The closest related taxa to isolate GSD1FS based on results from the EZBioCloud database were subsp. ATCC 25527, subsp. DSM 10140 and LMG 30189, belonging to the phylogenetic group. The resulting 16S rRNA gene identities (compared length of 1454 nucleotides) towards these taxa were 97.30, 97.23 and 97.09 %, respectively. The pairwise similarities of strain GSD1FS using , , , 60, , , and gene fragments to all valid representatives of the phylogenetic group were in the concatenated range of 83.08-88.34 %. Phylogenomic analysis based on whole-genome methods such as average nucleotide identity revealed that bifidobacterial strain GSD1FS exhibits close phylogenetic relatedness (88.17 %) to LMG 10738. Genotypic characteristics and phylogenetic analyses based on nine molecular markers, as well as genomic and comparative phenotypic analyses, clearly proved that the evaluated strain should be considered as representing a novel species within the phylogenetic group named as sp. nov. (GSD1FS=DSM 105923=LMG 30345=CCM 8806).
一株果糖-6-磷酸磷酸酮醇酶阳性菌株（GSD1FS）从一只 3 周龄德国牧羊犬的粪便样本中分离得到。基于 EZBioCloud 数据库的结果，与分离株 GSD1FS 亲缘关系最近的分类群为 subsp. ATCC 25527、 subsp. DSM 10140 和 LMG 30189，均属于 进化群。与这些分类群的 16S rRNA 基因序列（比较长度为 1454 个核苷酸）的同源性分别为 97.30%、97.23%和 97.09%。利用 、 、 、 60 、 、 和 基因片段，菌株 GSD1FS 与 进化群所有有效代表的成对相似性在串联范围内为 83.08-88.34%。基于全基因组方法（如平均核苷酸同一性）的基因组分析表明，双歧杆菌株 GSD1FS 与 LMG 10738 具有密切的系统发育关系（88.17%）。基于 9 个分子标记的基因型特征和系统发育分析，以及基因组和比较表型分析，明确证明评估的菌株应被视为 进化群内的一个新种，命名为 sp. nov.（GSD1FS=DSM 105923=LMG 30345=CCM 8806）。
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