Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy.
GenProbio srl, Parma, Italy.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2288-2297. doi: 10.1099/ijsem.0.004032. Epub 2020 Feb 17.
Two strains, i.e., 2176B and 2177B, were isolated from Golden-Headed Lion Tamarin () and Goeldi's monkey (). Isolates were shown to be Gram-positive, non-motile, non-sporulating, facultative anaerobic and d-fructose 6-phosphate phosphoketolase-positive. Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including 60, B, J, G and C genes) and the core genome revealed that bifidobacterial strains 2176B and 2177B exhibit close phylogenetic relatedness to DSM 103139 and LMG 11041, respectively. Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized species. Thus, sp. nov. (2176B=LMG 31469=CCUG 73785) and sp. nov. (2177B=LMG 31471=CCUG 73786 are proposed as novel species.
从金头狮狨（）和金狮面狨（）中分离出两株菌，即 2176B 和 2177B。分离株被证明为革兰氏阳性、非运动性、非孢子形成、兼性厌氧和 d-果糖-6-磷酸磷酸酮酶阳性。基于 16S rRNA 序列、多位点序列（包括 60、B、J、G 和 C 基因）和核心基因组的系统发育分析表明，双歧杆菌菌株 2176B 和 2177B 与 DSM 103139 和 LMG 11041 分别具有密切的系统发育关系。进一步基于分离株基因组序列的基因分型结合表型分析，清楚地表明这些菌株与迄今为止公认的种的每种模式菌株都不同。因此，提议将新种命名为（2176B=LMG 31469=CCUG 73785）和（2177B=LMG 31471=CCUG 73786）。
