Bifidobacterium vansinderenii sp. nov., isolated from faeces of emperor tamarin (Saguinus imperator).

A novel Bifidobacterium strain, Tam10B, i.e. LMG 30126, was isolated from emperor tamarin (Saguinus imperator). Cells were Gram-positive, non-motile, non-sporulating, non-haemolytic, facultative anaerobic and fructose 6-phosphate phosphoketolase-positive. Phylogenetic analyses based on 16S rRNA genes as well as multilocus sequences (representing hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that Bifidobacterium Tam10B exhibited close phylogenetic relatedness to Bifidobacterium tissieri DSM 100201. Comparative analysis of 16S rRNA gene sequences confirmed the phylogenetic results showing the highest gene sequence identity with strain B. tissieri DSM 100201 (96.5 %). Furthermore, genotyping based on the genome sequence of Tam 10B, in combination with phenotypic analyses, clearly showed that strain Tam10B is distinct from each of the type strains of the so far recognized Bifidobacterium species. The type strain Tam10B (=LMG 30126=CCUG 70655) represents a novel species, for which the name Bifidobacteriumvansinderenii sp. nov is proposed.