Hoffmann M K
J Immunol. 1980 Nov;125(5):2076-81.
The differentiation of B cells in the in vitro PFC-response to red blood cell antigen proceeds through 2 phases. Antigen-reactive B cells acquire the ability to interact with helper T cells in the first phase. This phase is controlled by macrophages through a mediator that they release (Interleukin 1 ([Il-1]). B cells convert into antibody-secreting cells (PFC) in the second phase, which is controlled by helper T cells or by a mediator that they release (T cell-replacing factors [TRF]). This is demonstrated in experiments in which Il-1 increases the number of B cells capable of responding to T cell help. The majority of antigen-reactive B cells reaches that state of differentiation within 40 hr of incubation with Il-1. After this time, the response of B cells depends no longer on the presence of Il-1 but on the presence of T cells or TRF. The presented data suggest that antigen-primed helper T cells (but not unprimed T cells) induce the release of Il-1 by macrophages, thereby also influencing the early phase of B cell differentiation.
在体外针对红细胞抗原的PFC反应中，B细胞的分化过程分为两个阶段。在第一阶段，抗原反应性B细胞获得与辅助性T细胞相互作用的能力。此阶段由巨噬细胞通过其释放的一种介质（白细胞介素1 [Il-1]）来控制。在第二阶段，B细胞转化为抗体分泌细胞（PFC），这一阶段由辅助性T细胞或其释放的一种介质（T细胞替代因子 [TRF]）控制。在实验中已证实这一点，即Il-1可增加能够对T细胞辅助作出反应的B细胞数量。大多数抗原反应性B细胞在与Il-1孵育40小时内达到该分化状态。在此之后，B细胞的反应不再依赖于Il-1的存在，而是依赖于T细胞或TRF的存在。所呈现的数据表明，抗原致敏的辅助性T细胞（而非未致敏的T细胞）诱导巨噬细胞释放Il-1，从而也影响B细胞分化的早期阶段。
