从一个克隆的T细胞系产生B细胞生长促进活性物质（DL）BCGF及其对BCL1 B细胞肿瘤的检测
Production of a B cell growth-promoting activity, (DL)BCGF, from a cloned T cell line and its assay on the BCL1 B cell tumor.
作者信息
Swain S L, Dutton R W
出版信息
J Exp Med. 1982 Dec 1;156(6):1821-34. doi: 10.1084/jem.156.6.1821.
Culture supernatants from a long-term alloreactive T cell line, the Dennert line C.C3.11.75 (DL) contain a B cell-growth-promoting activity. This activity can be assayed on normal B cells or on the in vivo BCL1 tumor line. We have called this activity (DL)BCGF. This activity can be distinguished from the T cell-replacing factor activity we had earlier found in DL supernates [(DL)TRF], which is required together with IL2 for the B cell plaque-forming cell response to erythrocyte antigens. The (DL)BCGF can be absorbed on untreated or glutaraldehyde-fixed BCL1. This absorption does not remove (DL)TRF activity. The production of (DL)BCGF is greatly enhanced when DL is cultured with IL2-containing supernatants. Sublines or clones of DL (DL.B10 and DL.A4) have been obtained that make large amounts of (DL)BCGF in the absence of any stimulator cells or IL2. B cells from the Xid-deficient male (DBA/2 X CBA/N)F1 mice do not respond to (DL)BCGF.
长期同种异体反应性T细胞系（即德内特系C.C3.11.75，简称DL）的培养上清液含有促进B细胞生长的活性。这种活性可以用正常B细胞或体内BCL1肿瘤系进行检测。我们将这种活性称为（DL）BCGF。这种活性可与我们之前在DL上清液中发现的T细胞替代因子活性（即（DL）TRF）区分开来，（DL）TRF与白细胞介素2共同作用，才能使B细胞对红细胞抗原产生空斑形成细胞反应。（DL）BCGF可被未处理的或经戊二醛固定的BCL1吸附。这种吸附不会去除（DL）TRF活性。当DL与含白细胞介素2的上清液一起培养时，（DL）BCGF的产生会大大增强。已获得DL的亚系或克隆（DL.B10和DL.A4），它们在没有任何刺激细胞或白细胞介素2的情况下能大量产生（DL）BCGF。来自Xid缺陷雄性（DBA/2×CBA/N）F1小鼠的B细胞对（DL）BCGF无反应。
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