Department of Pharmaceutical Technology.
Department of Surgery.
Eur J Gastroenterol Hepatol. 2021 Feb 1;32(2):178-185. doi: 10.1097/MEG.0000000000001875.
We aimed to determine the association of two of the most important functional polymorphisms of IL-8 and IL-10 with the clinical course and outcome of acute pancreatitis.
Ninety-three patients with acute pancreatitis were genotyped for IL-8-251T>A and IL-10-1082G>A using PCR-RFLP. The severity of the disease was determined based on the Atlanta Classification system.
In patients treated with opioids, the odds for severe form of acute pancreatitis, its complications, and death were increased. Advanced age was associated with higher odds of organ/multiple organ failure and other systemic complications. Multivariate logistic regression analyses confirmed the observed effect of age and use of opioids, and revealed higher odds for the development of severe form of acute pancreatitis [P = 0.017, odds ratio (OR): 4.324, 95% confidence interval (CI): 1.305-14.323], its complications in general (P = 0.011, OR: 4.936, 95% CI: 1.442-16.897), pancreatic necrosis (P = 0.032, OR: 3.922, 95% CI: 1.122-13.707) and systemic inflammatory response syndrome (P = 0.037, OR: 3.838, 95% CI: 1.085-13.583) in the absence of IL-10-1082G>A variant allele. The effect of IL-8 -251T>A on acute pancreatitis severity or mortality was not detected.
Our study suggests the IL-10 -1082A allele as a protective factor in acute pancreatitis. Opioid analgesics treatment in acute pancreatitis is associated with severity, complications and mortality, while advanced age increases the risk of systemic complications.
我们旨在确定白细胞介素-8（IL-8）和白细胞介素-10（IL-10）两个最重要的功能多态性与急性胰腺炎的临床病程和结局的关联。
使用 PCR-RFLP 技术对 93 例急性胰腺炎患者的 IL-8-251T>A 和 IL-10-1082G>A 进行基因分型。根据亚特兰大分类系统确定疾病的严重程度。
在接受阿片类药物治疗的患者中，患严重型急性胰腺炎、其并发症和死亡的几率增加。高龄与发生器官/多器官衰竭和其他全身并发症的几率较高有关。多变量逻辑回归分析证实了观察到的年龄和使用阿片类药物的影响，并揭示了发生严重型急性胰腺炎的几率较高（P=0.017，优势比[OR]：4.324，95%置信区间[CI]：1.305-14.323）、总体并发症（P=0.011，OR：4.936，95%CI：1.442-16.897）、胰腺坏死（P=0.032，OR：3.922，95%CI：1.122-13.707）和全身炎症反应综合征（P=0.037，OR：3.838，95%CI：1.085-13.583）的几率增加，而不伴有 IL-10-1082G>A 变体等位基因。未检测到 IL-8-251T>A 对急性胰腺炎严重程度或死亡率的影响。
我们的研究表明 IL-10-1082A 等位基因是急性胰腺炎的保护因素。急性胰腺炎中阿片类药物镇痛治疗与严重程度、并发症和死亡率有关，而高龄增加了全身并发症的风险。
