Curtet C, Bourgoin C, Bohy J, Saccavini J C, Thédrez P, Akoka S, Tellier C, Chatal J F
INSERM U211, Faculté de Médecine, Nantes, France.
Int J Cancer Suppl. 1988;2:126-32. doi: 10.1002/ijc.2910410728.
Monoclonal antibodies (MAbs) 19-9 and 73-3 specific for human colon adenocarcinoma were labelled with a high number of gadolinium atoms. Twenty five DTPA were chelated per MAb, with only slight loss of immunoreactivity. The NMR contrast agent Gd-25 DTPA-MAb 19-9 or 73-3 ([Gd] 17 mumole/kg, [MAb] 60 microM) was injected into nude mice bearing human colon adenocarcinoma (SW948). Tumours were removed 24 hr after injection and T1 was measured in vitro. T1 relaxation time varied according to MAb specificity against tumour targets; T1 decreased 20% for MAb 19-9 and MAb 73-3 with SW948 tumour. Imaging was performed with this model. Very good contrast was obtained 24 hr after Gd-25 DTPA-MAb injection.
将针对人结肠腺癌的单克隆抗体（MAb）19-9和73-3用大量钆原子进行标记。每个单克隆抗体螯合25个二乙三胺五乙酸（DTPA），免疫反应性仅有轻微损失。将核磁共振造影剂钆-25 DTPA-单克隆抗体19-9或73-3（[钆]17微摩尔/千克，[单克隆抗体]60微摩尔）注射到携带人结肠腺癌（SW948）的裸鼠体内。注射后24小时取出肿瘤并在体外测量T1。T1弛豫时间根据单克隆抗体对肿瘤靶点的特异性而变化；对于SW948肿瘤，MAb 19-9和MAb 73-3使T1降低了20%。用该模型进行成像。在注射钆-25 DTPA-单克隆抗体后24小时获得了非常好的对比度。
