3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark-Parque de Ciencia e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal.
ICVS/3B's-PT Government Associate Laboratory, 4806-909 Braga/Guimarães, Portugal.
Molecules. 2023 Apr 12;28(8):3394. doi: 10.3390/molecules28083394.
Collagen is the major structural protein in extracellular matrix present in connective tissues, including skin, being considered a promising material for skin regeneration. Marine organisms have been attracting interest amongst the industry as an alternative collagen source. In the present work, Atlantic codfish skin collagen was analyzed, to evaluate its potential for skincare. The collagen was extracted from two different skin batches (food industry by-product) using acetic acid (ASColl), confirming the method reproducibility since no significant yield differences were observed. The extracts characterization confirmed a profile compatible with type I collagen, without significant differences between batches or with bovine skin collagen (a reference material in biomedicine). Thermal analyses suggested ASColl's native structure loss at 25 °C, and an inferior thermal stability to bovine skin collagen. No cytotoxicity was found for ASColl up to 10 mg/mL in keratinocytes (HaCaT cells). ASColl was used to develop membranes, which revealed smooth surfaces without significative morphological or biodegradability differences between batches. Their water absorption capacity and water contact angle indicated a hydrophilic feature. The metabolic activity and proliferation of HaCaT were improved by the membranes. Hence, ASColl membranes exhibited attractive characteristics to be applied in the biomedical and cosmeceutical field envisaging skincare.
胶原蛋白是细胞外基质的主要结构蛋白，存在于结缔组织中，包括皮肤，被认为是皮肤再生的有前途的材料。海洋生物作为胶原蛋白的替代来源引起了业界的兴趣。在本工作中，分析了大西洋鳕鱼皮胶原蛋白，以评估其在护肤方面的潜力。使用乙酸（ASColl）从两个不同的皮批（食品工业副产品）中提取胶原蛋白，由于没有观察到产率的显著差异，因此证实了该方法的重现性。提取物的特性分析证实了与 I 型胶原蛋白兼容的特征，批次之间或与牛皮胶原蛋白（生物医学中的参考材料）之间没有显着差异。热分析表明，ASColl 的天然结构在 25°C 时丢失，并且热稳定性低于牛皮胶原蛋白。在角质细胞（HaCaT 细胞）中，ASColl 的细胞毒性直到 10mg/mL 都没有发现。ASColl 用于制备膜，其表面光滑，批次之间在形态或生物降解性方面没有显着差异。其吸水率和水接触角表明具有亲水性。膜改善了 HaCaT 的代谢活性和增殖。因此，ASColl 膜具有应用于生物医学和化妆品领域的有吸引力的特性，可用于护肤。
