从西伯利亚人群中两名健康个体的外周血单个核细胞中生成诱导多能干细胞系 ICGi021-A 和 ICGi022-A。
Generation of induced pluripotent stem cell lines ICGi021-A and ICGi022-A from peripheral blood mononuclear cells of two healthy individuals from Siberian population.
机构信息
Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia.
Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia.
出版信息
Stem Cell Res. 2020 Oct;48:101952. doi: 10.1016/j.scr.2020.101952. Epub 2020 Aug 8.
ICGi021-A and ICGi022-A iPSC lines were obtained by reprogramming PBMCs of two healthy women of the Siberian population using episomal non-integrating vectors expressing Yamanaka factors. iPSC lines expressed pluripotency markers, had a normal karyotype and demonstrated the ability to differentiate into derivatives of the three germ layers. Clinical exome sequencing data of the original biosamples of the donors are available in the NCBI SRA database. The generated cell lines are useful as "healthy" control in biomedical studies.
ICGi021-A 和 ICGi022-A 人 iPSC 系通过重编程两个来自西伯利亚人群的健康女性的 PBMC，使用表达山中因子的类病毒非整合载体获得。iPSC 系表达多能性标志物，具有正常核型，并表现出分化为三个胚层衍生物的能力。供体原始生物样本的临床外显子组测序数据可在 NCBI SRA 数据库中获得。所产生的细胞系可用作生物医学研究中的“健康”对照。
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