Department of Orthopaedics Shanghai Jiao Tong University Affiliated Sixth People's Hospital 600 Yishan Road, Shanghai 200233, China.
Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China.
Mater Sci Eng C Mater Biol Appl. 2020 Nov;116:111166. doi: 10.1016/j.msec.2020.111166. Epub 2020 Jun 6.
Posttraumatic peritendinous adhesion leads to limb disability. Physical barrier was widely used and thus focus was paid to fabricate the hydrophobic surfaces of electrospun membrane for anti-adhesion. However, current methods are limited and complicated. In this study, beeswax (Wax)/poly-L-lactic acid (PLA) anti-adhesion membranes were fabricated by blending electrospinning of Wax and PLA. The water contact angle was tested to investigate the hydrophobicity of the surfaces. Incorporation of Wax into PLA did not destroy the micro-pores between Wax/PLA fibers. After 7-day culture, proliferation of fibroblasts on Wax/PLA anti-adhesion membranes were significantly less than that on culture dish and PLA membranes. In rat Achilles adhesion model, least histological peritendinous adhesion formation was detected on the repaired sites in the group treated with Wax/PLA membranes than PLA membranes. Consequently, blending electrospinning of Wax into PLA is an easy method to fabricate hydrophobic surface of electrospun membrane with advanced peritendinous anti-adhesion outcome.
创伤后肌腱周围粘连可导致肢体残疾。物理屏障被广泛应用，因此人们专注于制造用于防粘连的电纺膜的疏水面。然而，目前的方法有限且复杂。在这项研究中，通过混合电纺蜡和聚 L-乳酸（PLA）制备了蜂蜡（Wax）/聚 L-乳酸（PLA）防粘连膜。通过测试水接触角来研究表面的疏水性。蜡的掺入并没有破坏蜡/PLA 纤维之间的微孔。培养 7 天后，成纤维细胞在 Wax/PLA 防粘连膜上的增殖明显少于培养皿和 PLA 膜上的增殖。在大鼠跟腱粘连模型中，与 PLA 膜相比，用 Wax/PLA 膜处理的修复部位的腱周粘连形成最少。因此，将蜡混合电纺到 PLA 中是一种简单的方法，可以制造具有先进腱周防粘连效果的电纺膜疏水面。
