Li Peichuang, Li Xiaojing, Cai Wanhao, Chen Huiqing, Chen Hang, Wang Rui, Zhao Yuancong, Wang Jin, Huang Nan
Key Lab. of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
Institute of Physical Chemistry, Albert-Ludwigs-Universität Freiburg, Albertstraße 23a, 79104 Freiburg, Germany.
Mater Sci Eng C Mater Biol Appl. 2020 Nov;116:111237. doi: 10.1016/j.msec.2020.111237. Epub 2020 Jun 23.
As an important class of biomaterials，bionics inspired materials has been widely used in creating extracorporeal and implantable medical devices. However, specific service environment is often faced with multiple requirements rather than single function. Herein, we designed a phospholipid-based multifunctional coating with phospholipids-based polymers, type I collagen (Col-I) and Arg-Glu-Asp-Val (REDV) peptide, via layer-by-layer assembly. The successful synthesis of the polymers and the coating is proved by a series of characterization methods including Fourier transforming infrared spectra (FTIR), proton nuclear magnetic resonance (H NMR), ultraviolet-visible spectra (UV) and X-ray photoelectron spectroscopy (XPS), while the assembly process and quality change of the coating were monitored via quartz crystal microbalance (QCM). Besides, hydrophilicity and roughness of this coating was analyzed via water contact angle (WCA) and atomic force microscope (AFM), respectively. Finally, results from platelet adhesion, activation assay, smooth muscle cells (SMCs) and endothelial cells (ECs) cultures indicated that the multifunctional coating could strongly inhibit platelet adhesion and SMCs proliferation, hence provide practical application of the coating with good biocompatibility, especially the anticoagulant property and cell compatibility. It is expected that this coating may be used in blood-contacting fields such as cardiovascular stent or other devices in the future.
作为一类重要的生物材料，仿生材料已被广泛应用于制造体外和可植入的医疗设备。然而，特定的使用环境通常面临多种要求而非单一功能。在此，我们通过层层组装设计了一种基于磷脂的多功能涂层，该涂层含有基于磷脂的聚合物、I型胶原蛋白（Col-I）和精氨酸-谷氨酸-天冬氨酸-缬氨酸（REDV）肽。通过包括傅里叶变换红外光谱（FTIR）、质子核磁共振（H NMR）、紫外可见光谱（UV）和X射线光电子能谱（XPS）在内的一系列表征方法证明了聚合物和涂层的成功合成，同时通过石英晶体微天平（QCM）监测涂层的组装过程和质量变化。此外，分别通过水接触角（WCA）和原子力显微镜（AFM）分析了该涂层的亲水性和粗糙度。最后，血小板粘附、活化测定、平滑肌细胞（SMCs）和内皮细胞（ECs）培养的结果表明，该多功能涂层可强烈抑制血小板粘附和平滑肌细胞增殖，从而为具有良好生物相容性，特别是抗凝性能和细胞相容性的涂层提供了实际应用。预计该涂层未来可用于诸如心血管支架或其他设备等与血液接触的领域。
