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通过电驱动紫外光和葡萄糖感应监测发酵的新可能性。

A New Possibility for Fermentation Monitoring by Electrical Driven Sensing of Ultraviolet Light and Glucose.

机构信息

School of Sciences and Engineering, Av. Domingos da Costa Lopes, São Paulo State University (Unesp), 780 Jardim Itaipu, CEP 17602-496 Tupã, SP, Brazil.

São Carlos Institute of Physics, University of São Paulo-Box 369, 13566-970, São Carlos, SP, Brazil.

出版信息

Biosensors (Basel). 2020 Aug 12;10(8):97. doi: 10.3390/bios10080097.

Abstract

Industrial fermentation generates products through microbial growth associated with the consumption of substrates. The efficiency of industrial production of high commercial value microbial products such as ethanol from glucose (GLU) is dependent on bacterial contamination. Controlling the sugar conversion into products as well as the sterility of the fermentation process are objectives to be considered here by studying GLU and ultraviolet light (UV) sensors. In this work, we present two different approaches of SnO nanowires grown by the Vapor-Liquid-Solid (VLS) method. In the GLU sensor, we use SnO nanowires as active electrodes, while for the UV sensor, a nanowire film was built for detection. The results showed a wide range of GLU sensing and as well as a significant influence of UV in the electrical signal. The effect of a wide range of GLU concentrations on the responsiveness of the sensor through current-voltage based on SnO nanowire films under different concentration conditions ranging was verified from 1 to 1000 mmol. UV sensors show a typical amperometric response of SnO nanowires under the excitation of UV and GLU in ten cycles of 300 s with 1.0 V observing a stable and reliable amperometric response. GLU and UV sensors proved to have a promising potential for detection and to control the conversion of a substrate into a product by GLU control and decontamination by UV control in industrial fermentation systems.

摘要

工业发酵通过与基质消耗相关的微生物生长来生产产品。从葡萄糖(GLU)生产高商业价值的微生物产品,如乙醇的工业生产效率取决于细菌污染。通过研究 GLU 和紫外线 (UV) 传感器,控制糖转化为产品以及发酵过程的无菌性是这里需要考虑的目标。在这项工作中,我们提出了两种通过气相-液相-固相 (VLS) 方法生长的 SnO 纳米线的不同方法。在 GLU 传感器中,我们使用 SnO 纳米线作为活性电极,而对于 UV 传感器,构建了纳米线薄膜进行检测。结果表明,GLU 传感器具有广泛的 GLU 感应范围,以及 UV 对电信号的显著影响。通过在 1 到 1000 mmol 不同浓度条件下基于 SnO 纳米线薄膜的电流-电压对传感器响应的 GLU 浓度的广泛范围进行了验证。UV 传感器在 UV 和 GLU 的激发下显示出 SnO 纳米线的典型电流响应,在 1.0 V 下观察到 10 个 300 s 的循环中有稳定可靠的电流响应。GLU 和 UV 传感器被证明在工业发酵系统中具有检测和控制底物转化为产物的潜力,通过 GLU 控制和 UV 控制进行去污染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a1d/7459838/aa475a4b455e/biosensors-10-00097-g001.jpg

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