Greither Thomas, Koser Franziska, Holzhausen Hans-Jürgen, Güttler Antje, Würl Peter, Kappler Matthias, Wach Sven, Taubert Helge
Center for Reproductive Medicine and Andrology, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.
Institute of Physiology II, University of Muenster, 48149 Münster, Germany.
Cancers (Basel). 2020 Aug 12;12(8):2254. doi: 10.3390/cancers12082254.
Soft tissue sarcoma (STS) is a heterogeneous group of rare malignancies with a five-year survival rate of approximately 50%. Reliable molecular markers for risk stratification and subsequent therapy management are still needed. Therefore, we analyzed the prognostic potential of miR-155-5p and miR-203a-3p expression in a cohort of 79 STS patients. MiR-155-5p and miR-203a-3p expression was measured from tumor total RNA by qPCR and correlated with the demographic, clinicopathological, and prognostic data of the patients. Elevated miR-155-5p expression was significantly associated with increased tumor stage and hypoxia-associated mRNA/protein expression. High miR-155-5p expression and low miR-203a-3p expression, as well as a combination of high miR-155-5p and low miR-203a-3p expression, were significantly associated with poor disease-specific survival in STS patients in the Kaplan-Meier survival analyses ( = 0.027, = 0.001 and = 0.0003, respectively) and in the univariate Cox regression analyses (RR = 1.96; = 0.031; RR = 2.59; = 0.002 and RR = 4.76; = 0.001, respectively), but not in the multivariate Cox regression analyses. In conclusion, the oncomiR miR-155-5p and the tumor suppressor-miR miR-203a-3p exhibit an association with STS patient prognosis and are suggested as candidates for risk assessment.
软组织肉瘤（STS）是一组异质性罕见恶性肿瘤，五年生存率约为50%。目前仍需要用于风险分层及后续治疗管理的可靠分子标志物。因此，我们分析了79例STS患者队列中miR-155-5p和miR-203a-3p表达的预后潜力。通过qPCR从肿瘤总RNA中检测miR-155-5p和miR-203a-3p的表达，并将其与患者的人口统计学、临床病理和预后数据相关联。miR-155-5p表达升高与肿瘤分期增加及缺氧相关mRNA/蛋白表达增加显著相关。在Kaplan-Meier生存分析中（分别为=0.027、=0.001和=0.0003）以及单因素Cox回归分析中（RR=1.96；=0.031；RR=2.59；=0.002和RR=4.76；=0.001），高miR-155-5p表达和低miR-203a-3p表达以及高miR-155-5p和低miR-203a-3p表达的组合与STS患者的疾病特异性生存不良显著相关，但在多因素Cox回归分析中并非如此。总之，癌基因miR-155-5p和肿瘤抑制性miR miR-203a-3p与STS患者预后相关，并被建议作为风险评估的候选指标。
