León-López Arely, Pérez-Marroquín Xóchitl Alejandra, Campos-Lozada Gieraldin, Campos-Montiel Rafael G, Aguirre-Álvarez Gabriel
Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Avenida Universidad Kilometro 1, Tulancingo C.P. 43600, Hidalgo, Mexico.
Uni-Collagen S.A. de C.V., Arnulfo González No. 203, El Paraíso, Tulancingo C.P. 43684, Hidalgo, Mexico.
Foods. 2020 Aug 12;9(8):1106. doi: 10.3390/foods9081106.
In this study, the preparation of a milk whey-based beverage with the addition of different concentrations of hydrolyzed collagen (0.3%, 0.5%, 0.75%, and 1%) was carried out. The control was considered at a concentration of 0%. Physicochemical properties, viscosity, antioxidant activity, and microbiological parameters were evaluated. The 1% collagen treatment showed the highest protein content (9.75 ± 0.20 g/L), as well as radical inhibition for ATBS (48.30%) and DPPH (30.06%). There were no significant differences ( ≥ 0.05) in the fat and lactose parameters. However, the pH in the control treatment was lower compared to beverages treated with hydrolyzed collagen. Fourier transform-infrared spectroscopy showed spectra characteristic of lactose and collagen amides. The viscosity increased significantly as the concentration of hydrolyzed collagen increased. The addition of hydrolyzed collagen increased the bioavailability, nutritional value, and the antioxidant activity of the beverage. Hydrolyzed collagen acted as an antimicrobial agent, as there was no presence of microorganism pathogens observed in the treated beverages.
在本研究中,制备了添加不同浓度水解胶原蛋白(0.3%、0.5%、0.75%和1%)的乳清基饮料。对照组的浓度为0%。对其理化性质、粘度、抗氧化活性和微生物参数进行了评估。1%胶原蛋白处理组的蛋白质含量最高(9.75±0.20 g/L),对ABTS的自由基抑制率为48.30%,对DPPH的自由基抑制率为30.06%。脂肪和乳糖参数无显著差异(≥0.05)。然而,对照组处理的pH值低于水解胶原蛋白处理的饮料。傅里叶变换红外光谱显示了乳糖和胶原蛋白酰胺的光谱特征。随着水解胶原蛋白浓度的增加,粘度显著增加。添加水解胶原蛋白提高了饮料的生物利用度、营养价值和抗氧化活性。水解胶原蛋白起到了抗菌剂的作用,因为在处理过的饮料中未观察到微生物病原体。