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HepG2 耗尽培养基对干细胞多能性的行为改变。

Behavioral Changes in Stem-Cell Potency by HepG2-Exhausted Medium.

机构信息

Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.

Istituto Zooprofilattico Sperimentale della Sardegna, Via Vienna 2, 07100 Sassari, Italy.

出版信息

Cells. 2020 Aug 12;9(8):1890. doi: 10.3390/cells9081890.

DOI:10.3390/cells9081890
PMID:32806709
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7547384/
Abstract

Wharton jelly mesenchymal stem cells (WJ-MSCs) are able to differentiate into different cell lineages upon stimulation. This ability is closely related to the perfect balance between the pluripotency-related genes, which control stem-cell proliferation, and genes able to orchestrate the appearance of a specific phenotype. Here we studied the expression of stemness-related genes, epigenetic regulators (), miRNAs (, and ) related to stemness, exosomes, the cell-cycle regulators () and , and the senescence-associated genes (, and . Cells were cultured in the presence or absence of the human hepatocarcinoma cell line HepG2-exhausted medium, to evaluate changes in stemness, differentiation capability, and senescence sensibility. Our results showed the overexpression of and reduced levels of mRNA. Moreover, we observed a downregulation of , and a simultaneous overexpression of and . These findings suggest that WJ-MSCs are more likely to retain a stem phenotype and sometimes to switch to a highly undifferentiable proliferative-like behavior if treated with medium exhausted by human HepG2 cell lines.

摘要

牙髓间质干细胞（WJ-MSCs）在受到刺激时能够分化为不同的细胞谱系。这种能力与多能性相关基因之间的完美平衡密切相关，这些基因控制着干细胞的增殖，以及能够协调特定表型出现的基因。在这里，我们研究了与干细胞相关的基因、表观遗传调节剂（）、与干细胞相关的 miRNAs（、和）、外泌体、细胞周期调节剂（和）以及衰老相关基因（、和）的表达。我们将细胞培养在存在或不存在人肝癌细胞系 HepG2 耗尽培养基的情况下，以评估干细胞特性、分化能力和衰老敏感性的变化。我们的结果表明，和的 mRNA 表达上调，而 水平降低。此外，我们观察到 下调，和同时上调。这些发现表明，如果用人类 HepG2 细胞系耗尽的培养基处理，WJ-MSCs 更有可能保持干细胞表型，有时会转变为高度未分化的增殖样行为。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/11aa31e5ed28/cells-09-01890-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/cbb7163ab11f/cells-09-01890-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/5486e63794c7/cells-09-01890-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/c25263d13662/cells-09-01890-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/11aa31e5ed28/cells-09-01890-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/4fa6c827d75e/cells-09-01890-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/c74bab230b81/cells-09-01890-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/09ee3fbb8195/cells-09-01890-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/8f9bfc13adee/cells-09-01890-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/cbb7163ab11f/cells-09-01890-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/5486e63794c7/cells-09-01890-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/c25263d13662/cells-09-01890-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0bd/7547384/11aa31e5ed28/cells-09-01890-g008.jpg

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