National Cancer Centre/National Clinical Research Centre for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Tonghua Institute for Food and Drug Control, Tonghua, China.
Phytochem Anal. 2021 May;32(3):339-346. doi: 10.1002/pca.2980. Epub 2020 Aug 18.
Lung cancer has the highest mortality rate among the various types of cancer. Panax ginseng (C. A. Mey). is a popular anti-cancer herbal supplement. The quality control of ginseng is crucial to ensure its clinical efficacy. This study aimed to establish new quality control methods for ginseng and to identify its main active components responsible for lung cancer treatment.
Ultra-high-performance liquid chromatography (UPLC) was used to establish fingerprints of 18 batches of ginseng. CCK-8 test was performed to evaluate the inhibitory activity of ginseng on Lewis lung cancer (LLC) cells. The spectrum-effect relationship analysis of ginseng was assessed by canonical correlation analysis (CCA) and bioactivity validation.
Six common peaks were identified and the variation coefficients were determined. The 18 batches of ginseng inhibited the proliferation of LLC cells to different degrees, showing different half maximal inhibitory concentration (IC ) values. Spectrum-effect relationship analysis showed that ginsenoside Ro is the main anti-proliferative constituent of LLC cell.
Spectrum-effect relationship is suitable for quality control of ginseng used for lung cancer. It is also effective in discovering the active ingredients related to the clinical efficacy of traditional Chinese medicine.
肺癌是各种癌症中死亡率最高的一种。人参（C. A. Mey）是一种流行的抗癌草药补充剂。人参的质量控制对于确保其临床疗效至关重要。本研究旨在建立人参的新质量控制方法，并确定其治疗肺癌的主要活性成分。
采用超高效液相色谱（UPLC）建立了 18 批人参的指纹图谱。通过 CCK-8 试验评估人参对 Lewis 肺癌（LLC）细胞的抑制活性。采用典型相关分析（CCA）和生物活性验证对人参的谱效关系进行评估。
鉴定出 6 个共有峰，并确定了变异系数。18 批人参对 LLC 细胞的增殖有不同程度的抑制作用，表现出不同的半最大抑制浓度（IC ）值。谱效关系分析表明，人参皂苷 Ro 是 LLC 细胞增殖的主要抑制成分。
谱效关系适用于肺癌用人参的质量控制，也可有效发现与中药临床疗效相关的活性成分。
