State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
Department of Pharmacology for Chinese Materia Medica, China Pharmaceutical University, Nanjing 211198, People's Republic of China.
J Pharm Biomed Anal. 2014 Feb;89:76-82. doi: 10.1016/j.jpba.2013.10.030. Epub 2013 Nov 4.
Panax ginseng C.A. Meyer has been known as a valuable traditional Chinese medicines for thousands years of history. Ginsenosides, the main active constituents, exhibit prominent immunoregulation effect. The present study first describes a holistic method based on chemical characteristic and lymphocyte proliferative capacity to evaluate systematically the quality of P. ginseng in thirty samples from different seasons during 2-6 years. The HPLC fingerprints were evaluated using principle component analysis (PCA) and hierarchical clustering analysis (HCA). The spectrum-efficacy model between HPLC fingerprints and T-lymphocyte proliferative activities was investigated by principal component regression (PCR) and partial least squares (PLS). The results indicated that the growth of the ginsenosides could be grouped into three periods and from August of the fifth year, P. ginseng appeared significant lymphocyte proliferative capacity. Close correlation existed between the spectrum-efficacy relationship and ginsenosides Rb1, Ro, Rc, Rb2 and Re were the main contributive components to the lymphocyte proliferative capacity. This comprehensive strategy, providing reliable and adequate scientific evidence, could be applied to other TCMs to ameliorate their quality control.
人参（Panax ginseng C.A. Meyer）作为一种传统的中药已有数千年的历史。人参的主要活性成分——人参皂苷，具有显著的免疫调节作用。本研究首次描述了一种基于化学特征和淋巴细胞增殖能力的整体方法，用于系统评估 2-6 年不同季节的 30 个人参样品的质量。采用主成分分析（PCA）和层次聚类分析（HCA）对 HPLC 指纹图谱进行了评价。通过主成分回归（PCR）和偏最小二乘法（PLS）研究了 HPLC 指纹图谱与 T 淋巴细胞增殖活性之间的谱效关系。结果表明，人参皂苷的生长可以分为三个时期，从第五年 8 月开始，人参表现出显著的淋巴细胞增殖能力。谱效关系与人参皂苷 Rb1、Ro、Rc、Rb2 和 Re 密切相关，它们是促进淋巴细胞增殖能力的主要贡献成分。这种综合策略为改善中药的质量控制提供了可靠和充分的科学依据，也可应用于其他中药。
