Laboratory of Emerging Infectious Diseases, School of Medicine, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Curitiba, PR, 80215-901, Brazil.
Human Tissue Bank, Pontifícia Universidade Católica do Paraná, Curitiba, PR, 80215-901, Brazil.
Cell Tissue Bank. 2020 Dec;21(4):573-584. doi: 10.1007/s10561-020-09856-x. Epub 2020 Aug 18.
Residual chemicals that are presented during tissue processing in human tissue banks can be a risk for the allograft recipient. Determine the residual concentrations of the antibiotics and detergent used in the process of human decellularized tissue-engineered heart valves stored in isotonic saline solution up to 18 months. A total of 24 human decellularized allografts were stored in sterile sodium chloride and analyzed immediately after the decellularization process (0 months) and after storage for 6, 12, and 18 months, which includes the use of sodium dodecyl sulfate (SDS) and antibiotics (cefoxitin, vancomycin hydrochloride, lincomycin hydrochloride, polymyxin B sulfate). These valves were used for suitability tests, the zone of inhibition evaluation, and direct contact cytotoxicity assay. The stock solution from 32 valves was used for LC-MS/MS analysis of antibiotics and SDS. Tissue samples from decellularized valves showed a zone of inhibition formation for S. aureus and B. subtilis, suggesting the presence of an inhibitory molecule in the tissue. Cytotoxicity tests were negative. Polymyxin B, vancomycin, and SDS were detected and quantified in human decellularized aortic and pulmonary allografts during all periods of the study. There were no traces of residual cefoxitin and lincomycin in the tissue stock solution. We found residual concentrations of the antibiotics and detergent used in the process of human decellularized tissue-engineered heart valves stored in isotonic saline solution up to 18 months.
在人类组织库的组织处理过程中存在的残留化学物质可能会对同种异体移植物受体造成风险。确定在等渗盐溶液中储存的脱细胞组织工程心脏瓣膜的抗生素和清洁剂的残留浓度，时间长达 18 个月。总共储存了 24 个人类脱细胞同种异体移植物，在无菌氯化钠中储存，并在脱细胞处理后立即（0 个月）以及储存 6、12 和 18 个月后进行分析，其中包括使用十二烷基硫酸钠（SDS）和抗生素（头孢西丁、盐酸万古霉素、盐酸林可霉素、硫酸多粘菌素 B）。这些瓣膜用于适用性测试、抑菌圈评估和直接接触细胞毒性测定。从 32 个瓣膜中提取的储备溶液用于抗生素和 SDS 的 LC-MS/MS 分析。脱细胞瓣膜的组织样本显示对金黄色葡萄球菌和枯草芽孢杆菌形成抑菌圈，表明组织中存在抑制分子。细胞毒性测试为阴性。多粘菌素 B、万古霉素和 SDS 在研究的所有期间均在人类脱细胞主动脉和肺动脉同种异体移植物中被检测到并定量。组织储备溶液中没有残留的头孢西丁和林可霉素痕迹。我们发现，在等渗盐溶液中储存的脱细胞组织工程心脏瓣膜的抗生素和清洁剂的残留浓度在 18 个月内。
