Whetstone C A
U.S. Department of Agriculture, National Veterinary Services Laboratories, Ames, IA 50010.
Vet Microbiol. 1988 Jan;16(1):1-8. doi: 10.1016/0378-1135(88)90121-6.
Monoclonal antibodies were produced against the Mirandola strain of canine adenovirus Type 1 (CAV-1) and the Manhattan strain of canine adenovirus Type 2 (CAV-2). The monoclonal antibodies were used in vitro in virus neutralization (VN) assays and in indirect fluorescent antibody (IFA) tests to examine several strains of each virus type. Out of 36 monoclonal antibodies produced against the Mirandola strain, 18 were type-specific for CAV-1 by IFA and 13 of those neutralized the virus in vitro. The other 18 antibodies bound both CAV-1 and CAV-2 by IFA; however, 7 of those specifically neutralized only CAV-1. The 160 monoclonal antibodies made against the Manhattan strain of CAV-2 yielded 77 type-specific antibodies by IFA, of which 39 neutralized only CAV-2 in vitro. The remaining 83 antibodies recognized both CAV-1 and CAV-2 by IFA, with 3 of those neutralizing both viral types. The hemagglutination inhibition (HI) test was performed on a selected monoclonal antibody from each specificity group. Although Type 1 CAV could be readily differentiated from Type 2 CAV by using type-specific monoclonal antibodies in the IFA or VN tests, strains within each type could not be differentiated. This is the first report of neutralizing monoclonal antibodies for a mammalian adenovirus.
制备了针对犬腺病毒1型（CAV-1）米兰多拉株和犬腺病毒2型（CAV-2）曼哈顿株的单克隆抗体。这些单克隆抗体用于病毒中和（VN）试验和间接荧光抗体（IFA）试验的体外检测，以检测每种病毒类型的多个毒株。在针对米兰多拉株产生的36种单克隆抗体中，通过IFA检测有18种对CAV-1具有型特异性，其中13种能在体外中和该病毒。另外18种抗体通过IFA检测与CAV-1和CAV-2都结合；然而，其中7种仅特异性中和CAV-1。针对CAV-2曼哈顿株制备的160种单克隆抗体通过IFA检测产生了77种型特异性抗体，其中39种在体外仅中和CAV-2。其余83种抗体通过IFA检测识别CAV-1和CAV-2，其中3种中和两种病毒类型。对每个特异性组中选择的一种单克隆抗体进行了血凝抑制（HI）试验。尽管通过在IFA或VN试验中使用型特异性单克隆抗体可以很容易地将1型CAV与2型CAV区分开来，但每种类型内的毒株无法区分。这是关于哺乳动物腺病毒中和单克隆抗体的首次报道。
