Nishio O, Ooseto M, Takagi K, Yamasita Y, Ishihara Y, Isomura S
Department of Virology, Aichi Prefectural Institute of Public Health.
Microbiol Immunol. 1990;34(10):871-7. doi: 10.1111/j.1348-0421.1990.tb01065.x.
For detection and identification of enteric adenovirus (Ad) types 40 and 41 in stool specimens, enzyme-linked immunosorbent assay (ELISA) was developed with the use of three monoclonal antibodies: Ad group-specific, Ad40 type-specific, and Ad41 type-specific antibodies. Of 860 fecal samples from patients with acute gastroenteritis, 44 strains of Ad were isolated using Graham 293 cell cultures. Of these isolates, 20 were typed as Ad40, 18 were Ad41, and 6 were other Ads by neutralization tests with cell cultures. Results of the ELISA tests on these 860 fecal samples resulted in good agreement to those with the cell culture method. The ELISA tests using Ad type-specific monoclonal antibodies proved to be a specific and rapid technique for laboratory diagnosis of acute gastroenteritis caused by enteric Ads.
为了检测和鉴定粪便标本中的肠道腺病毒（Ad）40型和41型，利用三种单克隆抗体开发了酶联免疫吸附测定（ELISA）法：腺病毒属特异性抗体、Ad40型特异性抗体和Ad41型特异性抗体。在860份急性胃肠炎患者的粪便样本中，使用格雷厄姆293细胞培养法分离出44株腺病毒。通过细胞培养中和试验，这些分离株中，20株被鉴定为Ad40型，18株为Ad41型，6株为其他腺病毒。对这860份粪便样本进行ELISA检测的结果与细胞培养法的结果高度一致。使用腺病毒型特异性单克隆抗体的ELISA检测被证明是一种用于实验室诊断由肠道腺病毒引起的急性胃肠炎的特异性快速技术。
