FKBP 相关 ncRNA-mRNA 轴在乳腺癌中的作用。
FKBP-related ncRNA-mRNA axis in breast cancer.
机构信息
Department of Radiation Oncology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, China.
Surgical Intensive Care Unit, First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, 310016, China.
出版信息
Genomics. 2020 Nov;112(6):4595-4607. doi: 10.1016/j.ygeno.2020.08.017. Epub 2020 Aug 16.
Breast cancer (BC) is a disease with morbidity ranking the first of women worldwidely. In current study, 11 DE-miRNAs, consisting of four FKBP4 related DE-miRNAs and seven FKBP5 related DE-miRNAs, were screened. Four hundred and eighty two predicted lncRNAs were found for DE-miRNAs. Then, expression and prognostic results of nine of top 20 lncRNAs of BC were significantly identified. LINC00662 and LINC00963 expression were significantly associated with patients' overall survival (OS). Then, nine potential upstream transcription factors were identified in motifs of DE-miRNAs. Three hundred and twenty target genes were identified for GO annotation and KEGG pathway analysis, which were mainly enriched in cysteine-type endopeptidase activity involved in apoptotic process. Construction and analysis in PPI network showed that RAB7A was selected as a hub gene with the topest connectivity scores. Differential expression analysis of nine in top ten hub genes of BC were significantly identified. RAB7A and ARRB1 expression were significantly related with BC patients' OS.
乳腺癌（BC）是一种发病率在全球范围内位居女性之首的疾病。在本研究中，筛选出了 11 个 DE-miRNAs，其中包括 4 个 FKBP4 相关的 DE-miRNAs 和 7 个 FKBP5 相关的 DE-miRNAs。为 DE-miRNAs 找到了 482 个预测的 lncRNA。然后，显著鉴定了 BC 前 20 个 lncRNA 中排名前 9 的表达和预后结果。LINC00662 和 LINC00963 的表达与患者的总生存期（OS）显著相关。然后，在 DE-miRNAs 的基序中鉴定了 9 个潜在的上游转录因子。GO 注释和 KEGG 通路分析共鉴定到 320 个靶基因，主要富集在参与凋亡过程的半胱氨酸型内肽酶活性中。PPI 网络的构建和分析表明，RAB7A 被选为具有最高连接分数的枢纽基因。显著鉴定了 BC 前 10 个枢纽基因中 9 个的差异表达分析。RAB7A 和 ARRB1 的表达与 BC 患者的 OS 显著相关。
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