Freund D, Arendt J, Vollrath L
Cell Tissue Res. 1977 Jul 11;181(2):239-44. doi: 10.1007/BF00219983.
In the present study an attempt was made to demonstrate melatonin in the rat pineal gland by means of immunohistochemistry. The anitbody used was raised against 5-methoxy-N-acetyltryptophan which is chemically similar to melatonin. Specific fluorescence was demonstrable only in pineals from rats killed during the night, when melatonin formation is high. It was restricted to parenchymal cells lying in a marginal zone of the organ. These results are discussed in relation to a subdivision of the pineal parenchyma into cortical and medullary areas.
在本研究中，试图通过免疫组织化学方法在大鼠松果体中证实褪黑素的存在。所用抗体是针对5-甲氧基-N-乙酰色氨酸制备的，该物质在化学结构上与褪黑素相似。仅在夜间处死的大鼠的松果体中可检测到特异性荧光，此时褪黑素的合成量较高。荧光局限于位于松果体边缘区的实质细胞。结合松果体实质可分为皮质区和髓质区这一情况对这些结果进行了讨论。
