School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA.
Trends Biotechnol. 2020 Sep;38(9):976-989. doi: 10.1016/j.tibtech.2020.02.007. Epub 2020 Mar 19.
Combining the diverse chemical functionality of proteins with the predictable structural assembly of nucleic acids has enabled the creation of hybrid nanostructures for a range of biotechnology applications. Through the attachment of proteins onto or within nucleic acid nanostructures, materials with dynamic capabilities can be created that include switchable enzyme activity, targeted drug delivery, and multienzyme cascades for biocatalysis. Investigations of difficult-to-study biological mechanisms have also been aided by using DNA-protein assemblies that mimic natural processes in a controllable manner. Furthermore, advances that enable the recombinant production and intracellular assembly of hybrid nanostructures have the potential to overcome the significant manufacturing cost that has limited the use of DNA and RNA nanotechnology.
将蛋白质的多样化化学功能与核酸的可预测结构组装相结合，使得能够为各种生物技术应用创建杂交纳米结构。通过将蛋白质附着在核酸纳米结构上或内部，可以创建具有动态能力的材料，包括可切换的酶活性、靶向药物递送以及用于生物催化的多酶级联。通过使用 DNA-蛋白质组装以可控的方式模拟自然过程，也有助于研究难以研究的生物学机制。此外，能够实现杂交纳米结构的重组生产和细胞内组装的进展有可能克服限制 DNA 和 RNA 纳米技术使用的巨大制造成本。
